[Histonet] GLASSWARE CALIBRATION
Hi netters, Could someone please shed some light: 1. How is calibration for glassware performed on non-Class A glassware? 2. If Class A glassware is used but no certificate is located - does it still require calibration? Thanx again. S Kahn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GLASSWARE CALIBRATION
1ml of water =1g mass. could you use this to determine the degree of error between calibration and real volume? On Wed, Mar 28, 2012 at 10:51 AM, shehnaz khan shehnazs...@gmail.comwrote: Hi netters, Could someone please shed some light: 1. How is calibration for glassware performed on non-Class A glassware? 2. If Class A glassware is used but no certificate is located - does it still require calibration? Thanx again. S Kahn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] GLASSWARE CALIBRATION
Thank you for posting something that has to do with the business of Histotechnology rather than redneck jokes, my delete finger has blisters on it. . Let us have some meaningful discussions on the problems and solutions in today's histotechnology field. Barry From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of shehnaz khan [shehnazs...@gmail.com] Sent: Wednesday, March 28, 2012 3:51 AM To: histonet; histonet Subject: [Histonet] GLASSWARE CALIBRATION Hi netters, Could someone please shed some light: 1. How is calibration for glassware performed on non-Class A glassware? 2. If Class A glassware is used but no certificate is located - does it still require calibration? Thanx again. S Kahn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GLASSWARE CALIBRATION
Calibrating glassware is a most in analytical chemistry but is of lesser importance while preparing staining solutions that, at the end, are going to used to determine if a tissue component has been stained or not, and seldom subjected to a quantitative intensity determination. A (+) PAS-Schiff reaction is what is needed and the intensity can depend on the temperature of the reaction, the condition of the solution or the amounts of the (+) components in the tissue. With so many sources for intensity it is of little importance if your cylinder is reading exactly 100mL when you are preparing the solution or if that amount is ± 0.5mL, especially when human error has to be considered also at the moment of the preparation. You have to trust the manufacturer of your glass equipment and accept the calibration provided with each glassware as true. Mind that, in addition, those calibrations are usually made at 20ºC and I do not think that 20ºC is the room temperature in most laboratories. But, IF you want to calibrate some glassware, you will need an analytical balance (not a common piece of equipment), a good thermometer to determine the distilled water temperature, a table with the density of water at different temperatures and you have to fill your cylinder, pipette, or whatever glassware you want to calibrate to a certain mark and them weigh the water. Divide the result (in grams) between the density of the water at the temperature you made the measurement and you will get the value of the volume. Too much trouble, just trust the manufacturer of your glassware! René J. --- On Wed, 3/28/12, shehnaz khan shehnazs...@gmail.com wrote: From: shehnaz khan shehnazs...@gmail.com Subject: [Histonet] GLASSWARE CALIBRATION To: histonet histo...@pathology.swmed.edu, histonet histonet@lists.utsouthwestern.edu Date: Wednesday, March 28, 2012, 4:51 AM Hi netters, Could someone please shed some light: 1. How is calibration for glassware performed on non-Class A glassware? 2. If Class A glassware is used but no certificate is located - does it still require calibration? Thanx again. S Kahn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] GLASSWARE CALIBRATION
Glass glassware, including glass pipets, has always been accurate enough for my needs in making up histological stains. This is not (repeat NOT) true of pipettors with disposable tips: Some of my pipettors deliver exactly what they say they do, some deliver 50% more, and some deliver 3 times as much as their factory calibration claims. I calibrate my pipettors by weighing the average volume of water delivered in 5 trials and write the true volume on a tape label on the barrel of the pipettor. Antibody dilutions using 2 pipettors thus require a bit of calculation. Allen A. Smith Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 28, 2012 8:39 AM To: histonet; histonet; shehnaz khan Subject: Re: [Histonet] GLASSWARE CALIBRATION Calibrating glassware is a most in analytical chemistry but is of lesser importance while preparing staining solutions that, at the end, are going to used to determine if a tissue component has been stained or not, and seldom subjected to a quantitative intensity determination. A (+) PAS-Schiff reaction is what is needed and the intensity can depend on the temperature of the reaction, the condition of the solution or the amounts of the (+) components in the tissue. With so many sources for intensity it is of little importance if your cylinder is reading exactly 100mL when you are preparing the solution or if that amount is ± 0.5mL, especially when human error has to be considered also at the moment of the preparation. You have to trust the manufacturer of your glass equipment and accept the calibration provided with each glassware as true. Mind that, in addition, those calibrations are usually made at 20ºC and I do not think that 20ºC is the room temperature in most laboratories. But, IF you want to calibrate some glassware, you will need an analytical balance (not a common piece of equipment), a good thermometer to determine the distilled water temperature, a table with the density of water at different temperatures and you have to fill your cylinder, pipette, or whatever glassware you want to calibrate to a certain mark and them weigh the water. Divide the result (in grams) between the density of the water at the temperature you made the measurement and you will get the value of the volume. Too much trouble, just trust the manufacturer of your glassware! René J. --- On Wed, 3/28/12, shehnaz khan shehnazs...@gmail.com wrote: From: shehnaz khan shehnazs...@gmail.com Subject: [Histonet] GLASSWARE CALIBRATION To: histonet histo...@pathology.swmed.edu, histonet histonet@lists.utsouthwestern.edu Date: Wednesday, March 28, 2012, 4:51 AM Hi netters, Could someone please shed some light: 1. How is calibration for glassware performed on non-Class A glassware? 2. If Class A glassware is used but no certificate is located - does it still require calibration? Thanx again. S Kahn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] GLASSWARE CALIBRATION
Allen Any pipette calibration company can test and calibrate your pipettors so they are dispensing accurately and within specifications. The cost is not that significant. We have our pipettors calibrated quarterly but twice a year is good too. If you are using variable pipettors they should be calibrated at several volumes. For example a variable pipettor that ranges from 100 to 1000 ul should be measured and calibrated at 1000ul, 500ul and 100ul Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Wednesday, March 28, 2012 10:08 AM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GLASSWARE CALIBRATION Glass glassware, including glass pipets, has always been accurate enough for my needs in making up histological stains. This is not (repeat NOT) true of pipettors with disposable tips: Some of my pipettors deliver exactly what they say they do, some deliver 50% more, and some deliver 3 times as much as their factory calibration claims. I calibrate my pipettors by weighing the average volume of water delivered in 5 trials and write the true volume on a tape label on the barrel of the pipettor. Antibody dilutions using 2 pipettors thus require a bit of calculation. Allen A. Smith Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 28, 2012 8:39 AM To: histonet; histonet; shehnaz khan Subject: Re: [Histonet] GLASSWARE CALIBRATION Calibrating glassware is a most in analytical chemistry but is of lesser importance while preparing staining solutions that, at the end, are going to used to determine if a tissue component has been stained or not, and seldom subjected to a quantitative intensity determination. A (+) PAS-Schiff reaction is what is needed and the intensity can depend on the temperature of the reaction, the condition of the solution or the amounts of the (+) components in the tissue. With so many sources for intensity it is of little importance if your cylinder is reading exactly 100mL when you are preparing the solution or if that amount is ± 0.5mL, especially when human error has to be considered also at the moment of the preparation. You have to trust the manufacturer of your glass equipment and accept the calibration provided with each glassware as true. Mind that, in addition, those calibrations are usually made at 20ºC and I do not think that 20ºC is the room temperature in most laboratories. But, IF you want to calibrate some glassware, you will need an analytical balance (not a common piece of equipment), a good thermometer to determine the distilled water temperature, a table with the density of water at different temperatures and you have to fill your cylinder, pipette, or whatever glassware you want to calibrate to a certain mark and them weigh the water. Divide the result (in grams) between the density of the water at the temperature you made the measurement and you will get the value of the volume. Too much trouble, just trust the manufacturer of your glassware! René J. --- On Wed, 3/28/12, shehnaz khan shehnazs...@gmail.com wrote: From: shehnaz khan shehnazs...@gmail.com Subject: [Histonet] GLASSWARE CALIBRATION To: histonet histo...@pathology.swmed.edu, histonet histonet@lists.utsouthwestern.edu Date: Wednesday, March 28, 2012, 4:51 AM Hi netters, Could someone please shed some light: 1. How is calibration for glassware performed on non-Class A glassware? 2. If Class A glassware is used but no certificate is located - does it still require calibration? Thanx again. S Kahn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
