Re: [Histonet] H pylori and Ventana instruments
We use Ventana's HP antibody, and we do see some of the small, non-specific globs of stain, but the pathologists are comfortable differentiating between the true and false staining since they are familiar with this artefact. Roger Heyna Rae Staskiewicz raest...@grics.net 5/7/2013 12:56 PM Hi all, Those of you using Ventana instrumentation, could you tell me what H pylori antibody you are using, and whether or not you are experiencing non-specific staining, or staining that looks specific but with a stippled effect. Thanks Rae Staskiewicz UnityPoint Health-Methodist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] H pylori and Ventana instruments
I haven't used HP, but have noticed this stippled effect especially when using 40X on the microscope and looking at slides labeled with an Ventana alk-phos kit. I seem to have solved the problem by making sure no trace of H2O is left on the slides. It helps after washing slides to run them thru a few changes of alcohol then one acetone and then a couple xylene before coverslipping. Ventana suggested the acetone step. Tom T -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Wednesday, May 08, 2013 8:41 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] H pylori and Ventana instruments We use Ventana's HP antibody, and we do see some of the small, non-specific globs of stain, but the pathologists are comfortable differentiating between the true and false staining since they are familiar with this artefact. Roger Heyna Rae Staskiewicz raest...@grics.net 5/7/2013 12:56 PM Hi all, Those of you using Ventana instrumentation, could you tell me what H pylori antibody you are using, and whether or not you are experiencing non-specific staining, or staining that looks specific but with a stippled effect. Thanks Rae Staskiewicz UnityPoint Health-Methodist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H pylori and Ventana instruments
Hi all, Those of you using Ventana instrumentation, could you tell me what H pylori antibody you are using, and whether or not you are experiencing non-specific staining, or staining that looks specific but with a stippled effect. Thanks Rae Staskiewicz UnityPoint Health-Methodist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] H pylori and Ventana instruments
Rae, We use Ventana's HP antibody with no problems. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rae Staskiewicz Sent: Tuesday, May 07, 2013 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H pylori and Ventana instruments Hi all, Those of you using Ventana instrumentation, could you tell me what H pylori antibody you are using, and whether or not you are experiencing non-specific staining, or staining that looks specific but with a stippled effect. Thanks Rae Staskiewicz UnityPoint Health-Methodist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
