RE: [Histonet] HE preparation

2009-09-02 Thread Weems, Joyce

 the good ole days for sure - purple ceilings, purple walls, purple
people And cigarette ashes in the trash and food on the counter...

Is it Friday yet?!!! 
J:)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette
Pavelich
Sent: Wednesday, September 02, 2009 07:02
To: aaz...@hotmail.com; histonet@lists.utsouthwestern.edu;
kemlo.roger...@waht.swest.nhs.uk
Subject: RE: [Histonet] HE preparation

Ah, the good 'ol days!  Remember adding the oxidizinng agent (mecuric
oxide) too soon when it was still bubbling and having a volcano of
hematoxylin?!!!  LOLj Was remembering tho', that every day, before
using, we would top off
(add...oh, about 30ml) of fresh to the filtered used batch.  This really
kept the quality good.  Worked for 30 yrs until we started purchasing
it.  Gone are the morning coffee and donuts sitting next to our
microtomes too!! Sigh  Ya, yaI know!!!  
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[Histonet] HE preparation

2009-09-01 Thread Aazath Raj

Dear All,
  We are preparing harris heamtoxylin for HE in our laboratory. some 
time we get intense nuclear staining some time light weak.Does anybody know how 
to control a consistency in the strength .


Aazath
Technical Officer
Apollo Hospitals
India

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Re: [Histonet] HE preparation

2009-09-01 Thread Rene J Buesa
The hematoxylin has to be ripen, either naturally (it takes long time) or with 
an oxidizer.
René J.

--- On Tue, 9/1/09, Aazath Raj aaz...@hotmail.com wrote:


From: Aazath Raj aaz...@hotmail.com
Subject: [Histonet] HE preparation
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 1, 2009, 11:48 AM



Dear All,
          We are preparing harris heamtoxylin for HE in our laboratory. some 
time we get intense nuclear staining some time light weak.Does anybody know how 
to control a consistency in the strength .


Aazath
Technical Officer
Apollo Hospitals
India

_
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http://in.msn.com___
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Histonet@lists.utsouthwestern.edu
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