Re: [Histonet] HSV1 and HSV2
Thank you for the information. I will look into your antibody source. Martha -Original Message- From: Cartun, Richard Sent: Tuesday, June 15, 2021 3:26 PM To: Martha Ward-Pathology Cc: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] RE: HSV1 and HSV2 Hi Martha: We are using type-specific monoclonals from BioSB (Santa Barbara, CA). They also sell a cocktail of the two antibodies which is what we use for screening. Rarely, do we get requests for type-specific identification. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) richard.car...@hhchealth.org -Original Message- From: Martha Ward-Pathology via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, June 15, 2021 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HSV1 and HSV2 CAUTION: This email is from outside HHC. USE CARE when opening attachments or links. We have always offered these two antibodies separately but recently we have begun having issues with our HSV2. In researching a new source I am seeing that some places do some sort of cocktail mixture of the two. What are others out there doing for this? Thanks in advance for your help. Martha Ward, Manager Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!PM-BBy3bF7epr83ucPrDa0D21BtxagGkNt2mKWWY7SUaExNwbpL43czZRyw5F0A-LMQ$ Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HSV1 and HSV2
Hi Martha: We are using type-specific monoclonals from BioSB (Santa Barbara, CA). They also sell a cocktail of the two antibodies which is what we use for screening. Rarely, do we get requests for type-specific identification. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) richard.car...@hhchealth.org -Original Message- From: Martha Ward-Pathology via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, June 15, 2021 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HSV1 and HSV2 CAUTION: This email is from outside HHC. USE CARE when opening attachments or links. We have always offered these two antibodies separately but recently we have begun having issues with our HSV2. In researching a new source I am seeing that some places do some sort of cocktail mixture of the two. What are others out there doing for this? Thanks in advance for your help. Martha Ward, Manager Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!PM-BBy3bF7epr83ucPrDa0D21BtxagGkNt2mKWWY7SUaExNwbpL43czZRyw5F0A-LMQ$ Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HSV1 and HSV2
We have always offered these two antibodies separately but recently we have begun having issues with our HSV2. In researching a new source I am seeing that some places do some sort of cocktail mixture of the two. What are others out there doing for this? Thanks in advance for your help. Martha Ward, Manager Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HSV1 and HSV2
For the immunologic pAB cocktail we are using Protease 1 (4 min) and ab incubation of 32 min. The only problem is that mast cells are also staining, it is a side effect of the protease 1. (AB concentration 1:400) Willem Hoekert OLVG Amsterdam The Netherlands Van: histonet-boun...@lists.utsouthwestern.edu namens Evans, Andria B Verzonden: vr 23-12-2011 18:48 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] HSV1 and HSV2 I am currently having issues with our HSV1 and HSV2 staining tissue components that it shouldn't be/background. We are running Ventana Benchmark XTs and Ultras. Using Dako's polyclonal rabbit concentrate. Does anyone out there in histoland have a concentration with a protocol that works and looks clean? Thank you for your help! Andria B Evans HTL(ASCP)CM This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HSV1 and HSV2
I am currently having issues with our HSV1 and HSV2 staining tissue components that it shouldn't be/background. We are running Ventana Benchmark XTs and Ultras. Using Dako's polyclonal rabbit concentrate. Does anyone out there in histoland have a concentration with a protocol that works and looks clean? Thank you for your help! Andria B Evans HTL(ASCP)CM This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
