Re: [Histonet] Help for identifying the blue stained structure
Hi Rui, You uploaded a tif image and a number of browsers don't support tif images. Jpeg, gif and png images are the best image formats to use because they are universally supported. I converted your image to a jpeg image and posted it at: http://histosearch.com/imageupload/help-for-identifying-the-blue-stained-structure-jpeg/ Best Regards, Marvin Hanna On 05/17/2014 06:39 PM, Rui TAHARA wrote: Hello, I have an embryonic sample that decalcified, paraffin embedded, and stained with Mallory Trichrome (Aniline Blue, Orange G, Acid Fuchsin). I will upload the image in the Histonet Images. This is a cranial region where the bone is being resorbed, so I expected to see the bone (dark blue in trabecular), and red blood, and adjacent white spaces that is being resorbed. Instead, there is a very uniform, granular structure stained with blue without any nuclei at the resorbed regions. This structure looks like almost crystal or some kind of secretion leakage from the ossifying bone. I need a help to identify this blue stained things. I don't think this is osteoid, because at later stage embryos, there is no bone at this region. If you have some suggestions for other stain to identify this blue thing, or help identifying this, I really appreciate it. Thank you in advance, Rui ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Help for identifying the blue stained structure
Hi, Thank you so much. I was wondering why the image has not been seen on the site, and thought its been processed. Thank you for uploading the image. Rui Date: Sun, 18 May 2014 17:47:21 -0400 From: mha...@histosearch.com To: ru...@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help for identifying the blue stained structure Hi Rui, You uploaded a tif image and a number of browsers don't support tif images. Jpeg, gif and png images are the best image formats to use because they are universally supported. I converted your image to a jpeg image and posted it at: http://histosearch.com/imageupload/help-for-identifying-the-blue-stained-structure-jpeg/ Best Regards, Marvin Hanna On 05/17/2014 06:39 PM, Rui TAHARA wrote: Hello, I have an embryonic sample that decalcified, paraffin embedded, and stained with Mallory Trichrome (Aniline Blue, Orange G, Acid Fuchsin). I will upload the image in the Histonet Images. This is a cranial region where the bone is being resorbed, so I expected to see the bone (dark blue in trabecular), and red blood, and adjacent white spaces that is being resorbed. Instead, there is a very uniform, granular structure stained with blue without any nuclei at the resorbed regions. This structure looks like almost crystal or some kind of secretion leakage from the ossifying bone. I need a help to identify this blue stained things. I don’t think this is osteoid, because at later stage embryos, there is no bone at this region. If you have some suggestions for other stain to identify this blue thing, or help identifying this, I really appreciate it. Thank you in advance, Rui ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help for identifying the blue stained structure
Hello, I have an embryonic sample that decalcified, paraffin embedded, and stained with Mallory Trichrome (Aniline Blue, Orange G, Acid Fuchsin). I will upload the image in the Histonet Images. This is a cranial region where the bone is being resorbed, so I expected to see the bone (dark blue in trabecular), and red blood, and adjacent white spaces that is being resorbed. Instead, there is a very uniform, granular structure stained with blue without any nuclei at the resorbed regions. This structure looks like almost crystal or some kind of secretion leakage from the ossifying bone. I need a help to identify this blue stained things. I don’t think this is osteoid, because at later stage embryos, there is no bone at this region. If you have some suggestions for other stain to identify this blue thing, or help identifying this, I really appreciate it. Thank you in advance, Rui ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
