If the CC1 standard 32 with amp, 42 degrees does not work then try using protease 1 4-6 minutes on it.
On Fri, Nov 28, 2008 at 12:35 PM, Lena Spencer <[EMAIL PROTECTED]>wrote: > Hi All: > > I have been working up the PMS2 and MLH2 mismatched repair genes and I am > not satisfied with my results. I am using the Cell Marque antibodies and > the Ventana XT using CC1, mild, standard and I have tried the antibodies > with block and amplification, and antibody times from 32 min- 1 hour and > the > stain is not satisfactory. Anyone who has these antibodies in their lab > and > would like to share your protocols, I would be grateful for your help. > The MSH2 and MSH6 are working great. > > Lena Spencer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- The Unknown HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet