That's the aluminum ammonium precipitating out, it happens with Harris also.
Do you filter it before use?
Histologistics
Hans B Snyder
508.308.7800
h...@histologistics.com
On Jan 15, 2015, at 16:46, Ingles Claire cing...@uwhealth.org wrote:
Hello All!
We have been having an interesting problem with our Hematoxylin. It seems
there are globules that form on the bottom of our hematoxylin wells in our
automatic stainer (Leica XL). These globs are acellular, so it is not a
fungus or something. We use a progressive Mayer's Hematoxylin formula as
follows:
1000ml DI
50g Aluminum Ammonium Sulfate
1g Hematoxylin powder
1g Citric Acid
0.2g Sodium Iodate
Anyone know what this might be? It doesn't affect the staining, but likes to
hang on the bottom of the staining racks and is a bit gross. Plus it gets
dragged into some of the following wells on occasion.
Thanks!
Claire
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