Karen, Regardless of the instrument you’re using, if related to a reagent, it may be that your detection chromogen is breaking down and you need to report it, or you have a material that is prepared onsite, and used onboard the instrument, and unknowingly contaminated.
it may help if you can provide an image. It can be done with a mobile phone up to the eyepiece if necessary. Best wishes, Eddie Martin The National Institutes of Health 301-594-2054 On Thu, Mar 23, 2023 at 1:00 PM <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. IHC gunk (Karen Heckford CA-San Francisco) > 2. Re: IHC gunk (Willis, Donna G) > 3. Re: IHC gunk (Thomas Podawiltz) > 4. Re: IHC gunk (Mac Donald, Jennifer) > 5. Re: Histonet Digest, Vol 232, Issue 7 (Eddie Martin) > > > > ---------- Forwarded message ---------- > From: Karen Heckford CA-San Francisco <karen.heckf...@commonspirit.org> > To: Histonet <histonet@lists.utsouthwestern.edu> > Cc: > Bcc: > Date: Wed, 22 Mar 2023 10:45:25 -0700 > Subject: [Histonet] IHC gunk > Good Morning, > I have recently developed a problem with contamination of some kind on my > IHC slides. > The contamination is black clumps and lays on top of the tissue. I have > been told it is bacteria but the Pathologist and I kind of doubt that. I > cannot get a good picture of it to show. It may not be on all the slides > on the same run or even the same antibody slide. > > I have decontaminated the whole system. I have put fresh reagents on the > instrument. This stuff looks like it would wash off at the end of the run > since it is sitting right on top of the tissue. I do not see this stuff > elsewhere on the slide, only the tissue. Does not matter if the tissue > was cut fresh or not. > > I have tried everything I can think of to get rid of it and still have this > issue. I use DiH20 from the hospital system. Not sure if this may be the > problem. I can have Engineering test the DiH20. > > Any help would be greatly appreciated. > > Thanks, > > Karen Heckford HT ASCP CE > > Lead Histology Technician > > St. Mary's Medical Center > > 450 Stanyan St. > > San Francisco, Ca. 94117 > > 415-750-5751 > > karen.heckford@ <karen.heckf...@dignityhealth.org>commonspirit.org > > > > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > Caution: This email is both proprietary and confidential, and not intended > for transmission to (or receipt by) any unauthorized person(s). If you > believe that you have received this email in error, do not read any > attachments. Instead, kindly reply to the sender stating that you have > received the message in error. Then destroy it and any attachments. Thank > you. > > > > > ---------- Forwarded message ---------- > From: "Willis, Donna G" <donna.wil...@bswhealth.org> > To: Karen Heckford CA-San Francisco <karen.heckf...@commonspirit.org>, > Histonet <histonet@lists.utsouthwestern.edu> > Cc: > Bcc: > Date: Wed, 22 Mar 2023 17:57:09 +0000 > Subject: Re: [Histonet] IHC gunk > What instrumentation are you using. Have you talked to your vendor? > > Donna Willis > Anatomic Pathology Manager > Baylor Scott&White Health > Baylor University Medical Center > 3500 Gaston Ave|Dallas, Texas 75246 > 214-820-2465 office|214-725-6184 mobile > > > > -----Original Message----- > From: Karen Heckford CA-San Francisco via Histonet < > histonet@lists.utsouthwestern.edu> > Sent: Wednesday, March 22, 2023 12:45 PM > To: Histonet <histonet@lists.utsouthwestern.edu> > Subject: {EXTERNAL} [Histonet] IHC gunk > > > CAUTION: This email originated outside of BSWH. The actual sender is ( > histonet-boun...@lists.utsouthwestern.edu) which may be different from > the display address in the From: field. Avoid action unless you know the > content is safe. Report suspicious emails using the PhishAlarm button > located in your Outlook ribbon. > > Good Morning, > I have recently developed a problem with contamination of some kind on my > IHC slides. > The contamination is black clumps and lays on top of the tissue. I have > been told it is bacteria but the Pathologist and I kind of doubt that. I > cannot get a good picture of it to show. It may not be on all the slides > on the same run or even the same antibody slide. > > I have decontaminated the whole system. I have put fresh reagents on the > instrument. This stuff looks like it would wash off at the end of the run > since it is sitting right on top of the tissue. I do not see this stuff > elsewhere on the slide, only the tissue. Does not matter if the tissue > was cut fresh or not. > > I have tried everything I can think of to get rid of it and still have > this issue. I use DiH20 from the hospital system. Not sure if this may be > the problem. I can have Engineering test the DiH20. > > Any help would be greatly appreciated. > > Thanks, > > Karen Heckford HT ASCP CE > > Lead Histology Technician > > St. Mary's Medical Center > > 450 Stanyan St. > > San Francisco, Ca. 94117 > > 415-750-5751 > > karen.heckford@ <karen.heckf...@dignityhealth.org>commonspirit.org > > > > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > Caution: This email is both proprietary and confidential, and not intended > for transmission to (or receipt by) any unauthorized person(s). If you > believe that you have received this email in error, do not read any > attachments. Instead, kindly reply to the sender stating that you have > received the message in error. Then destroy it and any attachments. Thank > you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!FwKdOPOzj_UZTnMVxT9qbcCJsUPm3F4e3bDrGK4DAjDyfuxQPVCf46_156AghOFHjVm2UeSVHSgv4hZS98GaWcNtyAQS7nLY$ > > ********************************************************************** > The information contained in this e-mail may be privileged, confidential, > and/or protected from disclosure. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you are > not the intended recipient (or have received this e-mail in error) please > notify the sender immediately and destroy this e-mail. Any unauthorized > copying, disclosure or distribution of the material in this e-mail is > strictly prohibited and no waiver of any attorney-client, work product, or > other privilege is intended. No binding agreement on behalf of Baylor Scott > & White Health, or any affiliated entity, is permitted by e-mail without > express written confirmation by a duly authorized representative of Baylor > Scott & White Health. > > > > ---------- Forwarded message ---------- > From: Thomas Podawiltz <tpodawi...@yahoo.com> > To: "Willis, Donna G" <donna.wil...@bswhealth.org>, Karen Heckford CA-San > Francisco <karen.heckf...@commonspirit.org>, Histonet < > histonet@lists.utsouthwestern.edu> > Cc: > Bcc: > Date: Wed, 22 Mar 2023 18:02:32 +0000 (UTC) > Subject: Re: [Histonet] IHC gunk > Is it on all slides or just certain antibodies? > > > Sent from Yahoo Mail for iPhone > > > On Wednesday, March 22, 2023, 1:57 PM, Willis, Donna G via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > What instrumentation are you using. Have you talked to your vendor? > > Donna Willis > Anatomic Pathology Manager > Baylor Scott&White Health > Baylor University Medical Center > 3500 Gaston Ave|Dallas, Texas 75246 > 214-820-2465 office|214-725-6184 mobile > > > > -----Original Message----- > From: Karen Heckford CA-San Francisco via Histonet < > histonet@lists.utsouthwestern.edu> > Sent: Wednesday, March 22, 2023 12:45 PM > To: Histonet <histonet@lists.utsouthwestern.edu> > Subject: {EXTERNAL} [Histonet] IHC gunk > > > CAUTION: This email originated outside of BSWH. The actual sender is ( > histonet-boun...@lists.utsouthwestern.edu) which may be different from > the display address in the From: field. Avoid action unless you know the > content is safe. Report suspicious emails using the PhishAlarm button > located in your Outlook ribbon. > > Good Morning, > I have recently developed a problem with contamination of some kind on my > IHC slides. > The contamination is black clumps and lays on top of the tissue. I have > been told it is bacteria but the Pathologist and I kind of doubt that. I > cannot get a good picture of it to show. It may not be on all the slides > on the same run or even the same antibody slide. > > I have decontaminated the whole system. I have put fresh reagents on the > instrument. This stuff looks like it would wash off at the end of the run > since it is sitting right on top of the tissue. I do not see this stuff > elsewhere on the slide, only the tissue. Does not matter if the tissue > was cut fresh or not. > > I have tried everything I can think of to get rid of it and still have > this issue. I use DiH20 from the hospital system. Not sure if this may be > the problem. I can have Engineering test the DiH20. > > Any help would be greatly appreciated. > > Thanks, > > Karen Heckford HT ASCP CE > > Lead Histology Technician > > St. Mary's Medical Center > > 450 Stanyan St. > > San Francisco, Ca. 94117 > > 415-750-5751 > > karen.heckford@ <karen.heckf...@dignityhealth.org>commonspirit.org > > > > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > Caution: This email is both proprietary and confidential, and not intended > for transmission to (or receipt by) any unauthorized person(s). If you > believe that you have received this email in error, do not read any > attachments. Instead, kindly reply to the sender stating that you have > received the message in error. Then destroy it and any attachments. Thank > you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!FwKdOPOzj_UZTnMVxT9qbcCJsUPm3F4e3bDrGK4DAjDyfuxQPVCf46_156AghOFHjVm2UeSVHSgv4hZS98GaWcNtyAQS7nLY$ > > ********************************************************************** > The information contained in this e-mail may be privileged, confidential, > and/or protected from disclosure. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you are > not the intended recipient (or have received this e-mail in error) please > notify the sender immediately and destroy this e-mail. Any unauthorized > copying, disclosure or distribution of the material in this e-mail is > strictly prohibited and no waiver of any attorney-client, work product, or > other privilege is intended. No binding agreement on behalf of Baylor Scott > & White Health, or any affiliated entity, is permitted by e-mail without > express written confirmation by a duly authorized representative of Baylor > Scott & White Health. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ---------- Forwarded message ---------- > From: "Mac Donald, Jennifer" <jmacdon...@mtsac.edu> > To: Karen Heckford CA-San Francisco <karen.heckf...@commonspirit.org>, > Histonet <histonet@lists.utsouthwestern.edu> > Cc: > Bcc: > Date: Wed, 22 Mar 2023 18:15:21 +0000 > Subject: Re: [Histonet] IHC gunk > Is it possible that it is hematoxylin precipitate? > > -----Original Message----- > From: Karen Heckford CA-San Francisco via Histonet < > histonet@lists.utsouthwestern.edu> > Sent: Wednesday, March 22, 2023 10:45 AM > To: Histonet <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] IHC gunk > > EXTERNAL SENDER - Exercise caution with requests, links, and attachments. > > Good Morning, > I have recently developed a problem with contamination of some kind on my > IHC slides. > The contamination is black clumps and lays on top of the tissue. I have > been told it is bacteria but the Pathologist and I kind of doubt that. I > cannot get a good picture of it to show. It may not be on all the slides > on the same run or even the same antibody slide. > > I have decontaminated the whole system. I have put fresh reagents on the > instrument. This stuff looks like it would wash off at the end of the run > since it is sitting right on top of the tissue. I do not see this stuff > elsewhere on the slide, only the tissue. Does not matter if the tissue > was cut fresh or not. > > I have tried everything I can think of to get rid of it and still have > this issue. I use DiH20 from the hospital system. Not sure if this may be > the problem. I can have Engineering test the DiH20. > > Any help would be greatly appreciated. > > Thanks, > > Karen Heckford HT ASCP CE > > Lead Histology Technician > > St. Mary's Medical Center > > 450 Stanyan St. > > San Francisco, Ca. 94117 > > 415-750-5751 > > karen.heckford@ <karen.heckf...@dignityhealth.org>commonspirit.org > > > > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > Caution: This email is both proprietary and confidential, and not intended > for transmission to (or receipt by) any unauthorized person(s). If you > believe that you have received this email in error, do not read any > attachments. Instead, kindly reply to the sender stating that you have > received the message in error. Then destroy it and any attachments. Thank > you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cjmacdonald%40mtsac.edu%7C27946f7c47f74e58436a08db2afd6592%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C638151040067352929%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=coq7k8kIhG03oke%2B477suyaDCCby1zEujMseWZ4lQSw%3D&reserved=0 > > > > > > ---------- Forwarded message ---------- > From: Eddie Martin <edmarti...@gmail.com> > To: histonet@lists.utsouthwestern.edu, "kdea...@hotmail.com" < > kdea...@hotmail.com> > Cc: > Bcc: > Date: Wed, 22 Mar 2023 15:47:53 -0400 > Subject: Re: [Histonet] Histonet Digest, Vol 232, Issue 7 > Hi kdea...@hotmail.com, > > What likely is occurring but you didn’t mention in the thread is that your > friend is using alkaline phosphatase based detection, which, by itself can > react with kidney tissue. This likely explains why your friend is getting > strong kidney staining and weak melan-a staining. > > This also tells me your friend didn’t optimize the antibody correctly. I’m > not sure why your friend is choosing to use a lyophilized antibody for > Melan-A as so many other commercially available options are available > capable of refrigerated storage and good up to 3 years. CellMarque makes an > Melan-A in both realty to use and/or a liquid concentrate with a 3-year > expiry date. > > Best wishes, > Eddie Martin, HTL,QIHC > The National Institutes of Health > Department of Laboratory Medicine > Bone Marrow Service > eddie.mar...@nih.gov > (301)-594-2054 > > > > On Wed, Mar 15, 2023 at 1:00 PM <histonet-requ...@lists.utsouthwestern.edu > > > wrote: > > > Send Histonet mailing list submissions to > > histonet@lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide Web, visit > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body 'help' to > > histonet-requ...@lists.utsouthwestern.edu > > > > You can reach the person managing the list at > > histonet-ow...@lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it is more specific > > than "Re: Contents of Histonet digest..." > > Today's Topics: > > > > 1. Problems with Melan A staining (Ken M) > > > > > > > > ---------- Forwarded message ---------- > > From: Ken M <kdea...@hotmail.com> > > To: "histonet@lists.utsouthwestern.edu" < > histonet@lists.utsouthwestern.edu > > > > > Cc: > > Bcc: > > Date: Wed, 15 Mar 2023 16:51:03 +0000 > > Subject: [Histonet] Problems with Melan A staining > > One of my histotech friends is having issues with Melan A staining. Using > > MA5-27949 Melan A antibody from Thermofisher, the negative Kidney tissue > > control was stained strongly positive while the positive melanoma tissue > > control was stained weakly positive. He couldn’t figure out why after > > several tries to reduce background. Can someone give me some suggestions? > > Thank you very much! > > > > > > Ken > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
