Hi Colleen,
I was involved in an Immunohistochemical study of the nerves in the human
ureter that used Zamboni's fixative and cryostat sections. This study was
before the invention of HIER.
If you are using Zamboni-fixed, paraffin embedded tissue then you will probably
need to do HIER before many of the localisation antibody incubations since the
fixative contains 2% formaldehyde. The same might be the case with some
antibodies when applied to cryostat sections.
Edyvane, K. A., Trussell, D. C., Jonavicius, J., Henwood, A., & Marshall, V. R.
(1992). Presence and regional variation in peptide-containing nerves in the
human ureter. Journal of the autonomic nervous system, 39(2), 127-137.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal
Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of
Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead
NSW 2145, AUSTRALIA
-Original Message-
From: Colleen Forster via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, 26 March 2021 9:42 AM
To: histonet-request
Subject: [Histonet] IHC with zamboni's fixed tissues
Hello Histonet,
Can you give me feedback on trying to do IHC on samples fixed in Zamboni's
fixative? I have an idea but need confirmation .
Thank you in advance
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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