Re: [Histonet] ISH Decal specimens

[Greg Dobbin via Histonet]

Use a formic acid based decal solution and MAKE SURE the specimen does not
fix (formalin fixation) longer than 32hrs (false negatives result). Use 70%
ETOH to manage the fixation time (ie fix for 24 hours, decal for 4 hrs,
place in 70% ETOH until it gets put on the processor. Hold in 70% ETOH for
weekends and holidays).
Greg
-- 
If God made a drink better than whiskey he kept it for himself! 弄
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Re: [Histonet] ISH decal specimens

[Val Tolley via Histonet]

Hi Kendra-

Decal Stat from Statlab is HCl based, therefore is not appropriate for 
subsequent IHC, ISH, or other molecular studies.  There is lots of 
peer-reviewed published data, if you need to supply rationale to your 
pathologists.  HCl is known to significantly reduce molecular assay sensitivity 
and cause false negatives.

Switch to either a formic-acid based decal (such as Immunocal) or EDTA-based 
decal and re-optimize your assay(s).

Val Tolley, HTL (ASCP), QIHC

> On Jun 23, 2023, at 8:53 AM, Kendra Beechie ND-Bismarck via Histonet 
>  wrote:
> 
> Hello all,
> 
> Recently, the Kappa/Lambda ISH has quit working on our decal specimens.
> When we first validated ISH, we tested decal specimens that stained
> appropriately. We use the DECAL Stat from StatLab. I did NOT do the initial
> ISH validation back in 2019, but as far as I know, we have not changed
> which Decal solution we use since then.
> 
> Has anyone else experienced this problem? I realize the other option is to
> run K/L IHC on the decal specimens, but we discontinued those after we
> brought the ISH on board. My medical director wants me to try to isolate
> the problem. My troubleshooting has only revealed that the decal is the
> common denominator.
> 
> Thanks for any help!
> 
> Caution: This email is both proprietary and confidential, and not intended 
> for transmission to (or receipt by) any unauthorized person(s). If you 
> believe that you have received this email in error, do not read any 
> attachments. Instead, kindly reply to the sender stating that you have 
> received the message in error. Then destroy it and any attachments. Thank you.
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[Histonet] ISH decal specimens

[Kendra Beechie ND-Bismarck via Histonet]

Hello all,

Recently, the Kappa/Lambda ISH has quit working on our decal specimens.
When we first validated ISH, we tested decal specimens that stained
appropriately. We use the DECAL Stat from StatLab. I did NOT do the initial
ISH validation back in 2019, but as far as I know, we have not changed
which Decal solution we use since then.

Has anyone else experienced this problem? I realize the other option is to
run K/L IHC on the decal specimens, but we discontinued those after we
brought the ISH on board. My medical director wants me to try to isolate
the problem. My troubleshooting has only revealed that the decal is the
common denominator.

Thanks for any help!

Caution: This email is both proprietary and confidential, and not intended for 
transmission to (or receipt by) any unauthorized person(s). If you believe that 
you have received this email in error, do not read any attachments. Instead, 
kindly reply to the sender stating that you have received the message in error. 
Then destroy it and any attachments. Thank you.
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