Re: [Histonet] Incomplete cross sections of all tissue in blocks
Steve A. McClain, MD at McClain Labs in Smithtown NY notes: >>...to avoid incomplete sectioning...: ink the specimens well, using acetic acid (vinegar) to fix the ink, thereby making the ink easier to see in the block.<< I don't like ink for this purpose, because it clutters up the microscopic section and interferes with photography. Better to use safranin O for this purpose (not fluorescent like eosin Y) - it isn't visible in the sections. The safranin solution used as a counterstain in the microbiology department's Gram stain works quite well. When I requested recuts for any reason, I'd always fetch the paraffin block myself and examine it. Saved many surprises. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Incomplete cross sections of all tissue in blocks
I suggest that each histotech is responsible for the blocks they cut and they cut the deepers on the their own blocks when they are requested. With feedback on the reason for the deeper from the pathologists they (the techs) will become more confident and learn how deep to cut. John On Tue, Mar 3, 2020 at 7:31 AM, Amy Self via Histonet wrote: > Good Morning HistoNetters, > > I am reaching out to the histonet in hopes to get some suggestions from you > on how to handle incomplete cross-sections of tissue in blocks. We are a > small lab so this has not been an issue in the past but now that we are > growing and our staff has increased I am getting feed-back from pathologist > that the sections of tissue are not complete. They are asking for too many > deepers that possibly could be avoided if it was cut deep enough to begin > with. I have been given some managerial type duties – which I don’t like > cause I know nothing about managing people and I need to approach this but I > need to approach this issue correctly. Do you have the histotech compare > his/her cut slides to the block to make sure that a complete cross-section is > obtained and is this documented somehow? Any and all suggestions I need. > > Thanks in advance for your help and as always you all rock.. 😊 > > Amy Self > Histology Lab Senior Tech > Lab > Tidelands Georgetown Memorial Hospital > 606 Black River Road > Georgetown, SC 29440 > (843) 520-8711 > as...@tidelandshealth.org > Our mission: We help people live better lives through better health. > > NOTE: > The information contained in this message may be privileged, confidential and > protected from disclosure. If the reader of this message is not the intended > recipient, or an employee or agent responsible for delivering this message to > the intended recipient, you are hereby notified that any dissemination, > distribution or copying of this communication is strictly prohibited. If you > have received this communication in error, please notify us immediately by > replying to this message and deleting it from your computer. > Thank you. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Incomplete cross sections of all tissue in blocks
Good Morning HistoNetters, I am reaching out to the histonet in hopes to get some suggestions from you on how to handle incomplete cross-sections of tissue in blocks. We are a small lab so this has not been an issue in the past but now that we are growing and our staff has increased I am getting feed-back from pathologist that the sections of tissue are not complete. They are asking for too many deepers that possibly could be avoided if it was cut deep enough to begin with. I have been given some managerial type duties – which I don’t like cause I know nothing about managing people and I need to approach this but I need to approach this issue correctly. Do you have the histotech compare his/her cut slides to the block to make sure that a complete cross-section is obtained and is this documented somehow? Any and all suggestions I need. Thanks in advance for your help and as always you all rock.. 😊 Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 (843) 520-8711 as...@tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
