[Histonet] Leica Coverslipper
Hi, The alerts are not from problems or malfunctions, they're from normal operation. For example the suction arm WILL alert if the cover glass is picked up and another is attached to the first one and somehow lands in a manner that makes the arm thinks the glass reservoir is empty. It WILL alert until someone quiets the alert, to let you know if the two racks are complete. It WILL alert once three racks have been coverslipped while it attempts to discharge a fourth rack and jam itself up. As someone mentioned previously, it definitely needs to be babysat just for normal operation. I agree that it is over-engineered , and yes the arm picking the slide up in the air is a poor design. The sakura uses an actuator to push the slide out and back in. In fact it's able to coverslip two slides in the same time the Leica does one. Although it is sufficient, It is not ideal for high volume operations. Personal preferences and contractual allegiances aside, the Sakura combo wins by a mile for bulletproof reliability and ability to handle large quantities without fail or alert. -- I have the Leica stainer and coverslipper, and I don't have anywhere near as many problems with the coverslipper as described by Contact below. Mine alerts once in a while; if his alerts that much, then something is seriously wrong. (The last time mine alerted that much, it needed a new brain-this is an older machine that had 5 circuit boards and one gave out-and one new sensor. Still worth it to us to fix it.) Anything as complex as staining and coverslipping robots will be fussy from time to time. But I love my Leica! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica Coverslipper
I can honestly say we have used it for years and do not baby-sit it. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Contact HistoCare Sent: Friday, March 22, 2013 10:30 AM To: kgrob...@rci.rutgers.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Coverslipper Hi, The alerts are not from problems or malfunctions, they're from normal operation. For example the suction arm WILL alert if the cover glass is picked up and another is attached to the first one and somehow lands in a manner that makes the arm thinks the glass reservoir is empty. It WILL alert until someone quiets the alert, to let you know if the two racks are complete. It WILL alert once three racks have been coverslipped while it attempts to discharge a fourth rack and jam itself up. As someone mentioned previously, it definitely needs to be babysat just for normal operation. I agree that it is over-engineered , and yes the arm picking the slide up in the air is a poor design. The sakura uses an actuator to push the slide out and back in. In fact it's able to coverslip two slides in the same time the Leica does one. Although it is sufficient, It is not ideal for high volume operations. Personal preferences and contractual allegiances aside, the Sakura combo wins by a mile for bulletproof reliability and ability to handle large quantities without fail or alert. -- I have the Leica stainer and coverslipper, and I don't have anywhere near as many problems with the coverslipper as described by Contact below. Mine alerts once in a while; if his alerts that much, then something is seriously wrong. (The last time mine alerted that much, it needed a new brain-this is an older machine that had 5 circuit boards and one gave out-and one new sensor. Still worth it to us to fix it.) Anything as complex as staining and coverslipping robots will be fussy from time to time. But I love my Leica! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica Coverslipper
We too have one that is a work horse. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Friday, March 22, 2013 9:35 AM To: Contact HistoCare; kgrob...@rci.rutgers.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica Coverslipper I can honestly say we have used it for years and do not baby-sit it. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Contact HistoCare Sent: Friday, March 22, 2013 10:30 AM To: kgrob...@rci.rutgers.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Coverslipper Hi, The alerts are not from problems or malfunctions, they're from normal operation. For example the suction arm WILL alert if the cover glass is picked up and another is attached to the first one and somehow lands in a manner that makes the arm thinks the glass reservoir is empty. It WILL alert until someone quiets the alert, to let you know if the two racks are complete. It WILL alert once three racks have been coverslipped while it attempts to discharge a fourth rack and jam itself up. As someone mentioned previously, it definitely needs to be babysat just for normal operation. I agree that it is over-engineered , and yes the arm picking the slide up in the air is a poor design. The sakura uses an actuator to push the slide out and back in. In fact it's able to coverslip two slides in the same time the Leica does one. Although it is sufficient, It is not ideal for high volume operations. Personal preferences and contractual allegiances aside, the Sakura combo wins by a mile for bulletproof reliability and ability to handle large quantities without fail or alert. -- I have the Leica stainer and coverslipper, and I don't have anywhere near as many problems with the coverslipper as described by Contact below. Mine alerts once in a while; if his alerts that much, then something is seriously wrong. (The last time mine alerted that much, it needed a new brain-this is an older machine that had 5 circuit boards and one gave out-and one new sensor. Still worth it to us to fix it.) Anything as complex as staining and coverslipping robots will be fussy from time to time. But I love my Leica! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica coverslipper and Ventana slides
I use histoclear in the last step of dehydration and have no problem with the arm picking up the slides. If I leave the slides for more than one hour in the last histoclear and if the histoclear covers the labels, then the glue of the label dissolves and interferes with the arm picking up the slides. Also If the labels are not places carefully on the slides and stick out from the slides then the arm has difficulty picking up the slides. Make sure your labels are placed carefully and use histoclear. If you have to use xylene make sure the xylene level is not very high so that It can affect the labels. 3 xylene changes 1 minute each is enough. Mesru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
