[Histonet] Lithium carbonate replacements for LFB
Help? !! Trying to do a LFB and don't have Li Carbonate to differentiate. Are there any other options? I have about two hours to figure it out. Please email me at the lab: cke...@pathgroupla.com Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax ad...@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to apn...@fullstaff.org. Please include your name and specialty in the body of the email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Lithium carbonate
Any chance it should have been 0.5 g lithium carbonate in 500 mL water? Maybe someone typed the label wrong? That's happened to us before. Is it supposed to be a 1.0% solution, or a 0.1% solution? Only reason I'm asking is that the only place we use lithium carbonate is in the LFB stain for myelin, and we use a 0.05% solution (0.25 g in 500 mL). If you are adding 5 g to 500 mL of water, that's a 1% solution, which, for lithium carbonate, is very close to being a saturated solution (where not all of it will dissolve). Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 (The opinions expressed are mine, and do not reflect on my hospital.) -Original Message- From: Nancy Schmitt Sent: Tuesday, November 15, 2011 8:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lithium carbonate Good Morning- Making up some lithium carbonate - it is staying very milky in appearance - have not previously had that happen. We tried a couple different times - same result. We are not doing anything different - 5g lithium carbonate to 500ml Type I water. Any thoughts? Thanks, Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Lithium carbonate
The solubility of Li2CO3 is 1.28% in cold water but only 0.71% in boiling water (data from the Merck Index). I've always kept on the lab shelf a bottle of saturated solution, with a little powder on the bottom. This keeps for ever and can be diluted 20-fold with water for such purposes as blueing haemalum or differentiating luxol fast blue. Lithium carbonate doesn't dissolve in alcohol, but that shouldn't be a problem. It's just a convenient, though rather expensive, source of not-too-alkaline water. John Kiernan Anatomy, UWO London, Canada = = = On 15/11/11, Nancy Schmitt wrote: > > Good Morning- > Making up some lithium carbonate - it is staying very milky in appearance - > have not previously had that happen. We tried a couple different times - > same result. We are not doing anything different - 5g lithium carbonate to > 500ml Type I water. > Any thoughts? > Thanks, Nancy > > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lithium carbonate
Good Morning- Making up some lithium carbonate - it is staying very milky in appearance - have not previously had that happen. We tried a couple different times - same result. We are not doing anything different - 5g lithium carbonate to 500ml Type I water. Any thoughts? Thanks, Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
