Hi,
SMAD4 is very difficult on the Ventana platform. The only clone that would work
on the Ventana to my knowledge(clone EP618Y, immunologic) has recently
'collapsed' (i hope that's the right word?) It means that it is not available
anymore. Maybe there is some vendor that has a few vials left, but my supplier
does not. If anybody knows of some other clone for the Ventana, I am very
interested.
MDM2: our sarcoma pathologist says that the MDM2 immuno is not reliable. Some
liposarcomas will be negative. It would be better to do FISH, which is also
very difficult because the fatty tissue often rinses off the slides.
Willem
Van: histonet-boun...@lists.utsouthwestern.edu namens Sue Hunter
Verzonden: do 5-12-2013 16:50
Aan: Histonet@lists.utsouthwestern.edu
Onderwerp: [Histonet] M2D2 and SMAD
Good morning
Does anyone have suggestions for antibodies for M2D2 and SMAD? We have not
been able to get these antibodies to work for us on the Ventana Ultras.
If you do have suggestions, protocols and control tissue suggestions would also
be appreciated.
Thanks
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edumailto:shun...@beaumont.edu
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