[Histonet] Microwave Decalcification

2015-05-22 Thread Huynh,Thomas
Hi Kimberly,

We are currently using the Milestone Medical brand microwave to decalcify 
our bone marrow core biopsies. After fixation we decal the core biopsies in the 
microwave for 2.5 hours at 50 degree Celsius with the 10% Formic Acid (we made 
up the formic acid in-house). If you need more info please email me.

Thanks 

Thomas  

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Today's Topics:

   1. Microwave Decalcification (Kimberly Marshall)
   2. Donating Old Microtome (Heather Marlatt)
   3. Re: Donating Old Microtome (Liette Tougas)
   4. Re: Donating Old Microtome (John Kiernan)
   5. Buying service contracts (Johnson, Carole)
   6. Re: Buying service contracts (Morken, Timothy)


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Message: 1
Date: Thu, 21 May 2015 17:25:04 +
From: Kimberly Marshall kimbe...@animalreferencepathology.com
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microwave Decalcification
Message-ID: 1432229164867.39...@animalreferencepathology.com
Content-Type: text/plain; charset=iso-8859-1

?Can anyone that has used the microwave decalcification method, please let me 
know how it worked for you?  Thanks in advance.


Kimberly



Kimberly Marshall H.T.(ASCP)

Histology/Lab Supervisor

Toll Free 1-800-426-2099

Fax 801-584-5104

PO Box 17580

Salt Lake City, Utah 84107

www.animalreferencepathology.comhttp://www.animalreferencepathology.com/



Advancing the art and science of veterinary medicine



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Message: 2
Date: Thu, 21 May 2015 12:49:48 -0700
From: Heather Marlatt hmarlat...@gmail.com
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Subject: [Histonet] Donating Old Microtome
Message-ID:
calavvk5jqsaf1b99eo0mxhjck4acgukqd+pkcnzsm_txzvn...@mail.gmail.com
Content-Type: text/plain; charset=UTF-8

I'm posting on behalf of a local researcher. They have been using an older 
microtome that was previously donated by a hospital. It broke down recently and 
they cant afford to replace it right now. It is used by the students to cut 
their own sections so they just need something that can section at 4 microns. 
They are happy to pay the freight if anyone out there has an old working 
microtome that they are willing to donate.

Thank you!!!

Heather


--

Message: 3
Date: Fri, 22 May 2015 03:24:09 +
From: Liette Tougas ltou...@dawsoncollege.qc.ca
To: Heather Marlatt hmarlat...@gmail.com,
histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Donating Old Microtome
Message-ID:
455897b94cf3a44f81f727160f23fb11640c5...@dc229.ad.dawsoncollege.qc.ca

Content-Type: text/plain; charset=iso-8859-1

Hi Heather, 

I do not know where you are located but we have 5-6 good old AO microtomes that 
still work very well that we are willing to donate.  They are in excellent 
condition as they were extremely well maintained over the years and, as a 
Biomed Lab technology teaching department, they have been used only twice a 
week for 3 weeks each fall semester since they were purchased, some 30 years 
ago!  They each have a disposable blade holder (high profile) and block clamp 
(vs original screwing block holder).  We easily cut at 4 microns on them. The 
offer is opened to anyone who can pick them up or pay for shipment.  

Liette Tougas, RT, B.Sc., M.Sc.
Biomedical Laboratory Technology Department Dawson College, Montr?al, Qc, 
Canada 514-931-8731, ext 1519 
From: Heather Marlatt [hmarlat...@gmail.com]
Sent: May 21, 2015 3:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Donating Old Microtome

I'm posting on behalf of a local researcher. They have been using an older 
microtome that was previously donated by a hospital. It broke down recently and 
they cant afford to replace it right now. It is used by the students to cut 
their own sections so they just need something that can section at 4 microns. 
They are happy to pay the freight if anyone out there has an old working 
microtome that they are willing to donate.

Thank you!!!

Heather

[Histonet] Microwave Decalcification

2010-07-23 Thread Figliuolo, Leticia
Hello all,

We inherited a Pelco Biowave 34700 (from Ted Pella) and would like to optimize 
decalcification of rats knee joints, dogs rib using the Biowave. I was 
wondering if anyone have a protocol for decalcification using nitric acid or 
formic acid or EDTA using microwave or if you know someone that uses microwave 
technology for decalcification that I can ask.

Your help is appreciated.
Leticia
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