[Histonet] Napsin A
Hello. I was wondering if anyone had used the NapsinA from BioCare on the Bond III and if so, would you mind sharing your protocol? Thanks in advance for any advice you can give. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] napsin a ccontrol slides
Hey Histonetters Where can I buy Napsin A control slides we don't have any adenocarcinoma's for internal ?? Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Napsin A controls
Hi Histonetters Are there any other controls for Napsin A other than Lung adenocarcinoma? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Napsin A controls
Kidney -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Wednesday, September 21, 2011 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Napsin A controls Hi Histonetters Are there any other controls for Napsin A other than Lung adenocarcinoma? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Napsin-A
I am starting to work up Napsin-A (purchased from Biocare Medical) on our Ventana instruments using iView DAB. Does anyone have a recommended protocol to get me started? Amanda Steier, HT(ASCP) Sanford Health Fargo, ND --- Please note that My Email Address Has Changed! Please begin using the address in the From line above, immediately. Soon, email sent to my old address will no longer be delivered to me. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain privileged and confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Napsin A
Hello Everyone, We are in the process of working up Napsin A. I was wondering what tissue others are using for multi tissue blocks to validate the anitbody? Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital, CT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Napsin A
We are using a Lung Adenocarcinoma as a daily control. Although I have noticed that normal lung macrophages will pick it up. We ran our validation of the antibody using a multi tumor block (contains various tumors from various organs) and a multi normal tissue block to ensure that it stained only the expected things and not anything else. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Piche [jhist...@yahoo.com] Sent: Monday, September 13, 2010 11:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Napsin A Hello Everyone, We are in the process of working up Napsin A. I was wondering what tissue others are using for multi tissue blocks to validate the anitbody? Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital, CT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Napsin A
Hi Loralee, Thanks for your response. What did you use for the multi normal tissue block? Kidney? Also for the multi-tumor block did you just use tumors from organs that you knew were negative for Napsin or did you use both positive and negative tumors? Thanks, Jessica From: McMahon, Loralee A loralee_mcma...@urmc.rochester.edu To: Jessica Piche jhist...@yahoo.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Mon, September 13, 2010 1:29:58 PM Subject: RE: [Histonet] Napsin A We are using a Lung Adenocarcinoma as a daily control. Although I have noticed that normal lung macrophages will pick it up. We ran our validation of the antibody using a multi tumor block (contains various tumors from various organs) and a multi normal tissue block to ensure that it stained only the expected things and not anything else. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Piche [jhist...@yahoo.com] Sent: Monday, September 13, 2010 11:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Napsin A Hello Everyone, We are in the process of working up Napsin A. I was wondering what tissue others are using for multi tissue blocks to validate the anitbody? Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital, CT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Napsin A
Hi Jessica, Our control for this antibody is normal lung, adenocarcinoma of the lung, and thyroid. We use this same block for other stains too (TTF-1, sufactant-A, EMA, etc.) but if I were going to make a control block specific for this antibody, I would want to include a squamous cell carcinoma of the lung to show that it's negative for napsin-A. You may see a very tiny amount of staining on the squamous cell carcinomas where the pulmonary-aveolar macrophages are eating up the keratin, but the tumor cells will be negative. Ovarian carcinomas can also stain. I would include a few of those too so the docs can see what that looks like. I would also make sure to include thyroid and thyroid carcinomas. With a particular vendor's antibody, napsin-a seems to stain thyroid (I don't want to name names), but with Novocastra's antibody it doesn't. It was helpful to our pathologists to have an antibody that doesn't stain thyroid. Thanks Mark On Mon, Sep 13, 2010 at 8:57 AM, Jessica Piche jhist...@yahoo.com wrote: Hello Everyone, We are in the process of working up Napsin A. I was wondering what tissue others are using for multi tissue blocks to validate the anitbody? Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital, CT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
