[Histonet] Necrotic and apoptotic cells

2010-09-24 Thread Amos Brooks 
Hi,
   Tunel is commonly used to show both apoptosis  necrosis. Caspase 3 is
good for Apoptosis. I just recently used a GR-1 antibody from AbCam that
shows a lot of promise for necrosis.

Amos



Message: 7
Date: Thu, 23 Sep 2010 19:35:24 +0100
From: Thotakura, Anil Kumar a.thotak...@imperial.ac.uk
Subject: [Histonet] Necrotic and apoptotic cells
To: Histonet@lists.utsouthwestern.edu
   Histonet@lists.utsouthwestern.edu
Message-ID: 
c8c15c7c.8e72%a.thotak...@imperial.ac.ukc8c15c7c.8e72%25a.thotak...@imperial.ac.uk

Content-Type: text/plain; charset=iso-8859-1

Dear All,

I want to do look for Necrotic and apoptotic cells on liver tumor sections.
What staining and antibodies are good for this technique?.

Please let me know. Thank you very much in advance.

Many Thanks,
Anil Kumar.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Necrotic and apoptotic cells

2010-09-23 Thread Thotakura, Anil Kumar 
Dear All,

I want to do look for Necrotic and apoptotic cells on liver tumor sections.
What staining and antibodies are good for this technique?.

Please let me know. Thank you very much in advance.

Many Thanks,
Anil Kumar.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Necrotic and apoptotic cells

2010-09-23 Thread Anatoli Gleiberman 
Anil,
For apoptotic cells in the liver you can use In situ cell death
detection kit, fluorescein (Roche, cat.#11 684 795 910) and use
counterstain with different color (Cy3, AlexaFluor 546 or Texas Red) for
the detection of endogenous IgG. Liver cells do not produce IgG, but
necrotic cells are usually impregnated with plasma proteins - and bright
staining for endogenous IgG is easy and reliable way to visualize such
cells. Works well on FFPE sections after citrate boiling retrieval.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: agleiber...@cbiolabs.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Thotakura, Anil Kumar
Sent: Thursday, September 23, 2010 2:35 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Necrotic and apoptotic cells 

Dear All,

I want to do look for Necrotic and apoptotic cells on liver tumor
sections.
What staining and antibodies are good for this technique?.

Please let me know. Thank you very much in advance.

Many Thanks,
Anil Kumar.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 


This communication may contain privileged information.  It is intended solely 
for the use of the addressee.  If you are not the intended recipient, you are 
strictly prohibited from disclosing, copying, distributing or using any of this 
information.  If you received this communication in error, please contact the 
sender immediately and destroy the material in its entirety, whether electronic 
or hard copy.  This communication may contain nonpublic information about 
individuals and businesses subject to the restrictions of the 
Gramm-Leach-Bliley Act.  You may not directly or indirectly reuse or redisclose 
such information for any purpose other than to provide the services for which 
you are receiving the information.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet