Re: [Histonet] Opinion on dye on biopsies
John Garratt notes: >>Be aware that validation of IHC should be performed if you are changing your processing protocol by adding a dye to the reagents or to a pre-processed tissue. Be cautious!<< I don't think safranin O would be a problem, but a good idea nonetheless! On Mon, Jan 14, 2019 at 6:40 PM John Garratt wrote: > Be aware that validation of IHC should be performed if you are changing > your processing protocol by adding a dye to the reagents or to a > pre-processed tissue. Be cautious! > > > John > > > > On Saturday, January 12, 2019 11:52 AM, Bob Richmond via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > Gareth Davis asked about dyes to use to mark small GI biopsy specimens to > > make sure they're recovered during embedding. > > > > I've had good results marking small specimens with the solution of > safranin > > O that's used in the microbiologists' Gram stain. Go to the micro lab and > > ask for a small amount of it and try it. > > > > Do not use eosin on biopsy specimens. Eosin's brilliant fluorescence > makes > > it very difficult to do any kind of fluorescent stain on the sections. > (It > > also doesn't work as well as safranin, which isn't fluorescent.) > > > > Another necessary procedure at the gross desk: fill out a log sheet that > > records the number of specimens you put into the cassette, and have that > > log sheet in front of you when you embed. (I've had a lot of histotechs > > flatly refuse to do this.) > > > > I like those little blue foam pads you put in the cassette and put the > > small specimens on. I usually cut them in two before putting them in the > > cassette. > > > > Bob Richmond > > Samurai Pathologist > > Maryville TN > > > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Opinion on dye on biopsies
Be aware that validation of IHC should be performed if you are changing your processing protocol by adding a dye to the reagents or to a pre-processed tissue. Be cautious! John On Saturday, January 12, 2019 11:52 AM, Bob Richmond via Histonet wrote: > Gareth Davis asked about dyes to use to mark small GI biopsy specimens to > make sure they're recovered during embedding. > > I've had good results marking small specimens with the solution of safranin > O that's used in the microbiologists' Gram stain. Go to the micro lab and > ask for a small amount of it and try it. > > Do not use eosin on biopsy specimens. Eosin's brilliant fluorescence makes > it very difficult to do any kind of fluorescent stain on the sections. (It > also doesn't work as well as safranin, which isn't fluorescent.) > > Another necessary procedure at the gross desk: fill out a log sheet that > records the number of specimens you put into the cassette, and have that > log sheet in front of you when you embed. (I've had a lot of histotechs > flatly refuse to do this.) > > I like those little blue foam pads you put in the cassette and put the > small specimens on. I usually cut them in two before putting them in the > cassette. > > Bob Richmond > Samurai Pathologist > Maryville TN > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Opinion on dye on biopsies
Gareth Davis asked about dyes to use to mark small GI biopsy specimens to make sure they're recovered during embedding. I've had good results marking small specimens with the solution of safranin O that's used in the microbiologists' Gram stain. Go to the micro lab and ask for a small amount of it and try it. Do not use eosin on biopsy specimens. Eosin's brilliant fluorescence makes it very difficult to do any kind of fluorescent stain on the sections. (It also doesn't work as well as safranin, which isn't fluorescent.) Another necessary procedure at the gross desk: fill out a log sheet that records the number of specimens you put into the cassette, and have that log sheet in front of you when you embed. (I've had a lot of histotechs flatly refuse to do this.) I like those little blue foam pads you put in the cassette and put the small specimens on. I usually cut them in two before putting them in the cassette. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Opinion on dye on biopsies
One article that might be useful is: Surprenant D, Garib G, Hutchens K, Dreifke M, Speiser J, Winterfield L, Peterson A, Krol C, Adams W, Tung R. Novel Use of Preoperative Epidermal Coloring of Very Small Dermatological Specimens-Protocol for Reduction of Lost Specimens. The American Journal of Dermatopathology. 2016 Jul 1;38(7):510-2. I would expect that this would colour the squamous cell layer of the oesophageal mucosa. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: Gareth Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, 10 January 2019 9:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinion on dye on biopsies So, I work in a small GI lab, and I put Eosin in my first formalin on my processor. My biopsies are very small and this helps, somewhat, to see the specimens for embedding and cutting. But, unfortunately, the esophagus tissues do not absorb the eosin much. Anyway, the hospital lab I work, part-time, in has started using hematoxylin to help see their biopsies. I happen to embed there and I think it just makes a big mess and the tissue does not absorb much of the stain. What are other labs doing to aid in making their small biopsies easier to see? What are pros and cons to doing this, in your opinion? Thanks! -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Opinion on dye on biopsies
So, I work in a small GI lab, and I put Eosin in my first formalin on my processor. My biopsies are very small and this helps, somewhat, to see the specimens for embedding and cutting. But, unfortunately, the esophagus tissues do not absorb the eosin much. Anyway, the hospital lab I work, part-time, in has started using hematoxylin to help see their biopsies. I happen to embed there and I think it just makes a big mess and the tissue does not absorb much of the stain. What are other labs doing to aid in making their small biopsies easier to see? What are pros and cons to doing this, in your opinion? Thanks! -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
