Good Morning to all: I am having staining problems with my PAX-5 predilute from CellMarque. I was wondering if anyone else has experienced a dramatic dropoff in staining signal with this antibody after a few months. I have now had this happen to me twice in the last 3 months. It stains very nicely when the vial is first opened and seems to hold staining intensity for about 3 months and then drops off dramatically even though the expiration date is 2 years out. I am using it on the Leica Bond. I have been in contact with CellMarque about this problem but they tell me they have not had any complaints from any other labs. I use many antibodies from CellMarque and have only seen this problem occur with one other antibody (ALK-1) about a year ago. If you are experiencing this problem in your lab please contact Liz Beitman at CellMarque (lbeit...@cellmarque.com<mailto:lbeit...@cellmarque.com>) so that they can have all available information to further research this problem. Thank you! Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.
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