change the tissues to 50%
alcohol at the grossing bench so that all tissues are not only fixed, but
rinsed in alcohol and started processing at the growing bench. This simplifies
processing to dehydration, clearing and paraffin infiltration. Simple is
reliable and short processing cycles on this machine leave little room for
error.
2) Program heat to 50C in all alcohols and xylene. Mixing pressure and vacuum
yes yes yes
4) Keep all solutions fresh, rotate after 200- 400 blocks.
5) Lay blocks out not more than 2 cassettes back to back, max 100 for VIP5.
4 hour is reliable for tissues up to 3mm thickness such as punch biopsy.
2 hour is reliable for up to 1.5 mm tissue thickness such as core biopsies and
shave biopsies of skin.
Method of processor validation for short cycle in machine previously validated
in your lab.
To validate create your evaluation form and find a set of cases with sufficient
tissue small tissue to divide into 2 equal blocks. This can (and will) be done
on clinical cases if you are careful.
Print duplicate sets of cassettes.
Mark one half of case 1 yellow and one half blue (tissues will be processed
separately, 1 regular and one short cycle and then recombined into 1 final
block at embedding).
We held the short processed tissue cassettes in formalin and timed the short
cycle to end when the regular program ends.
Then the two blocks are recombined or embedded together so that direct
comparisons/measures can be made on one H and one on your most common IHC for
that tissue. If the H and IHC both work, chances are everything else will
also.
Modify your regular validation form or Create a form for scoring shrinkage,
measured thickness and length, staining reactions, shrinkage, holes and flaws,
have your path score. photo graph yellow and blue tissues under H and IHC.
IF you do observe shrinkage or other undesirable artifact, be certain that your
tissues are completely fixed at grossing and repeat.
If they are comparable in the oculars and to the camera, then show your work
and voila, there is your validation.
Steve A. McClain, MD
>
--
Message: 7
Date: Fri, 8 Jan 2016 13:26:53 +
From: "Sanders, Jeanine (CDC/OID/NCEZID)" <j...@cdc.gov>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] lab humidifiers
Message-ID:
<3b2cd438e1628a41bd687e98b963b7813a5a9...@embx-clft4.cdc.gov>
Content-Type: text/plain; charset="us-ascii"
Morning everyone!
What is the standard in humidity levels that most histology labs strive for?
And to achieve this goal, do any of you use a commercial-grade humidifier?
Thanks for your help and have a nice weekend!
Jeanine H. Sanders
Centers for Disease Control and Prevention
1600 Clifton Rd., NE MS-G32
Atlanta, GA 30329
--
Message: 8
Date: Fri, 8 Jan 2016 13:49:18 +
From: Lester Raff MD <lr...@uropartners.com>
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Thanks for assistance
Message-ID:
<6347C6D2B080534F9B5C2B08436DCFAF0B5F2347@COLOEXCH01.uropartners.local>
Content-Type: text/plain; charset="us-ascii"
Thanks for spreading the word about block disposal. I have been inundated with
requests, will have to sort through them all!
For anyone interested Friday blog post
http://www.chicagonow.com/downsize-maybe/2016/01/what-is-the-last-sound-you-will-hear/
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
--
Message: 9
Date: Fri, 8 Jan 2016 14:42:33 + (UTC)
From: Histology Technician <histology81...@att.net>
To: Histonet List <histonet@lists.utsouthwestern.edu>,
"histonet-requ...@lists.utsouthwestern.edu"
<histonet-requ...@lists.utsouthwestern.edu>
Subject: [Histonet] Price gun
Message-ID:
<447350760.1678249.1452264153035.javamail.ya...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
? I was thinking of using a price gun to date my supplies when they come in,
but I can't find one to buy.? Any ideas where to search for one?What do you
guys use to date your supplies?? I've always used a pen to write the rcd date
and my initials but thought a price gun would be quicker and neater :)
Thanks!
--
Message: 10
Date: Fri, 8 Jan 2016 15:01:08 +
From: Michael Ann Jones <mjo...@metropath.com>
To: "Sanders, Jeanine (CDC/OID/NCEZID)" <j...@cdc.gov>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] lab humidifiers
Message-ID: <d2b51bc0.f59c%mjo...@metropath.com>
Content-Type: text/plain; charset="iso-8859-1"
We are struggling wit
