Hello Dorothy! I will forward you my protocol just as soon as I can get to my
computer.
On a side note, I no longer Chair the Hard Tissue Committee for the National
Society for Histotechnology (NSH) as I have now been elected to the NSH Board
of Directors as the Region III Director for the Southeast region. With that
said, Sarah Mack is now the new Hard Tissue Committee Chairperson and Kim
Simmons is the Education Development Manager for the NSH. I tell you this
because Sarah is located in your part of the country and is currently the
Histology Core Manager at University of Rochester Medical Center, Center for
Musculosketeal Research. She works with bone and will be an good resource for
you. I have copied her on this message. Kim Simmons will be helping to organize
bone related educational materials and events for the NSH in the next year so
she will be another good reference for you to follow. I have copied her to this
message as well. I will still be a part of the Hard Tissue Committee in a
supporting role. I just wanted you to know your additional options for
information related to the histology of bone, biomaterials and medical device
implants.
Best Regards,
Jack Ratliff
On Oct 22, 2014, at 3:20 PM, Dorothy Hu abt...@gmail.com wrote:
Dear Histoneters,
Can someone email me your TRAP protocol for MMA section? I have one, but
staining is not consistent. No matter I stain for how long, ~ several hours
sometimes. There are always variations. I sincerely hope I can get
different protocol form you and make change. I remember saw other TRAP
protocol which is much longer than mine.
The protocol I used was:
1. Dissolve 12mg Naphthol AS-MX phosphate (Sigma, N-5000) in 0.5ml N,N
methylformamide (Sigma,D8654).
2. Mix above into 50 ml Sodium tartrate with 0.1M sodium acetate buffer at
pH5.0.
3. Mix 30mg Fast Red Violet LB salt (Sigma, F3381) with above solution and
filter.
Stain slides @37oC for at least one hour. The solution can be use for one
month when stored in 4oC.
Continue to do counterstain and mount slides.
Thank you.
Dorothy Hu
MGH Endocrine histocore
Email: d...@partners.org
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