[Histonet] RE: B5 fixative

[Green JumpyOne]

Amy, 

We use the B+ fixative available from BBC Biochemical  ( 
http://www.bbcus.com/products.html?pc=19pid=57 )

My pathologists love it!

Michelle


 Message: 3
 Date: Tue, 02 Mar 2010 14:12:04 -0500
 From: Amy Farnan farn...@nehealth.com
 Subject: [Histonet] bone marrow fixative
 To: histonet@lists.utsouthwestern.edu
 Message-ID: 4b8d1cb4.26ed.00d...@nehealth.com
 Content-Type: text/plain; charset=US-ASCII
 
 Good afternoon everyone,
  
 In past years our lab used B-5 fixative on bone marrow biopsies for better 
 immunostaining.
 With the chemical hazards of B-5 we switched to AZF fixative and have been 
 using this for the past few years. I am just curious what everyone else is 
 using for fixative on their bone marrows and how is your immunoreactivity?  
 Maybe a better question is what is the preferred fixative for bone marrows?
  
 Have a nice day,
 Amy Farnan
 Histology Supervisor
 Northeast Health
 ***
  
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[Histonet] Re: B5 fixative

[Robert Richmond]

Well, this pathologist was dragged screaming away from B-5 - and I've
brewed plenty of it out of the dry chemicals myself - but you really
can't use it any more. I was surprised to find out that it's still
available, but it still is.

I haven't found any of the substitutes to be worthwhile. The major
advantage of B-5 was that it's a rapid fixative (for small specimens).
Lymphoid tissue and marrow really need to be fixed overnight in
neutral buffered formalin, for optimal fixation.

Bob Richmond
Samurai Pathologist
Knoxville TN

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