Rabbit anti-Phospho-Histone H2A.X (Ser139) Antibody #2577 from Cell Signaling works very well in FFPE(dilution 1:100 in immunofluorescence). Don't know good anti 53BP1.
Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Ellender Sent: Thursday, February 03, 2011 5:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 53BP1 and gamma-H2AX antibodies Good morning Histonet, Does anyone know of 53BP1 and gamma-H2AX antibodies that work in FFPE rat tissue (lung, liver, spleen, brain). Would appreciate any suggestions anyone might have, Best wishes, Michele Dr Michele Ellender Biological Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Health Protection Agency, Chilton, Didcot, Oxon. OX11 ORQ UK Phone: +44 (0)1235 822697 Fax: +44 (0)1235 833891 E-mail michele.ellen...@hpa.org.uk <mailto:michele.ellen...@hpa.org.uk> ----------------------------------------- ************************************************************************** The information contained in the EMail and any attachments is confidential and intended solely and for the attention and use of the named addressee(s). It may not be disclosed to any other person without the express authority of the HPA, or the intended recipient, or both. If you are not the intended recipient, you must not disclose, copy, distribute or retain this message or any part of it. This footnote also confirms that this EMail has been swept for computer viruses, but please re-sweep any attachments before opening or saving. HTTP://www.HPA.org.uk ************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
