[Histonet] RE: Antibody IDH1

2013-03-04 Thread Sue Hunter 
We use Dianova's antibody at a 1:100 dilution on the Ultra.  CC1 cell 
conditioning for 40 minutes, antibody for 32 minutes - no heat.  We use 
Optiview detection system. No amp.  Make sure you are using the correct type of 
brain tissue for a control - most oligodendrogliomas should be positive, altho 
there are a few with other mutations.  Remember that this antibody only picks 
up the R132H point mutation.  This occurs in about 70% of oligos so it is 
possible that you happened to pick one that was positive for one of the other 
mutations.  Perhaps try a few different patient tissues.

Sue


Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zimmerman, 
Billie
Sent: Friday, February 22, 2013 4:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody IDH1

Does anyone perform this antibody?? We can't get it to work on the Ventana 
Ultra.  PhenoPath in Seattle performs it on the Dako autostainer using a small 
size polymer detection kit.  I would appreciate any input.

Thanks,

Billie Zimmerman


Augusta State University and Georgia Health Sciences University have 
consolidated to become Georgia Regents University. Effective January 9, 2013, 
my email address has changed to bzimm...@gru.edu. Please update your address 
book to reflect this change.
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[Histonet] RE: Antibody IDH1

2013-02-22 Thread Bitting, Angela K. 
Are you using the antibody from Dianova??

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zimmerman, 
Billie
Sent: Friday, February 22, 2013 4:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody IDH1

Does anyone perform this antibody?? We can't get it to work on the Ventana 
Ultra.  PhenoPath in Seattle performs it on the Dako autostainer using a small 
size polymer detection kit.  I would appreciate any input.

Thanks,

Billie Zimmerman


Augusta State University and Georgia Health Sciences University have 
consolidated to become Georgia Regents University. Effective January 9, 2013, 
my email address has changed to bzimm...@gru.edu. Please update your address 
book to reflect this change.
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