This was years ago be we stained for the virus via IHC. The antibody was supplied by the client. That would seem to me to be the best method to determine deposits.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rodriguez, Kristofer Sent: Wednesday, October 22, 2014 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody deposit visualization One of my clients has been performing experiments with a mouse model of Dengue Virus Infection. They have been treating the mice with monoclonal antibodies (client did not specify what antibody)as a potential treatment for dengue virus infection. They have harvested the kidneys and put them in 10% NBF. They want to know if routine H&E will show any antibody deposits. I told him that I would not be able to directly observe the antibody deposit itself, however I might be able to observe the reaction to the antibody deposit in the basement membrane of the glomeruli as a membranous glomerulopathy on an H&E. I was wondering if I should do any special stains that might show more than an H&E. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
