[Histonet] RE: Biocare Decloaker Nx Gen
We recently bought this equipment and it works well. We are happy with it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, March 28, 2013 1:52 PM To: Swartwood, Steven J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Biocare Decloaker Nx Gen We have used the Decloaking Chamber for several years now and have had excellent results. The nicest thing is that there is consistency and reliability. Each run will be the same as the one previous and having the ability to use the USB stick for documentation of time, pressure and temperature makes record keeping really easy. We have had no complaints. The only con I can come up with is that it comes in a big box that is hard to store if you have to send it back for some reason. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Swartwood, Steven J Sent: Thursday, March 28, 2013 1:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare Decloaker Nx Gen Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope unmasking (heat retrieval)? Also does anyone have any personal pros/cons with this instrument? We are thinking about getting one because of inconsistencies with our current method using a vegetable steamer. We use many different tissue types both animal and human. Also we use many different antibodies. Currently with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure it wasn't due to fixation vimentin staining was performed and shows even staining. It's just certain antibodies that are giving us trouble even from multiple different companies. Any advice would be greatly appreciated and very beneficial to our work pertaining to the instrument. Thanks again everyone have a great end of the week, Steven Swartwood HT(ASCP) steven.swartw...@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Biocare Decloaker Nx Gen
I would welcome input hear as well since we are looking at buying one as well. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Swartwood, Steven J Sent: Thursday, March 28, 2013 12:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare Decloaker Nx Gen Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope unmasking (heat retrieval)? Also does anyone have any personal pros/cons with this instrument? We are thinking about getting one because of inconsistencies with our current method using a vegetable steamer. We use many different tissue types both animal and human. Also we use many different antibodies. Currently with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure it wasn't due to fixation vimentin staining was performed and shows even staining. It's just certain antibodies that are giving us trouble even from multiple different companies. Any advice would be greatly appreciated and very beneficial to our work pertaining to the instrument. Thanks again everyone have a great end of the week, Steven Swartwood HT(ASCP) steven.swartw...@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Biocare Decloaker Nx Gen
We tried the Decloaking Chamber Plus a couple years ago after using the Decloaking chamber for years. The Decloaking chamber gave us great results, but the record keeping (time, temp, pressure) for four Decloakers was time consuming and irritating. The PLUS model uses a USB stick to record all that so we didn't have to. A great improvement. But we ended up going with Bond stainers which obviated all that. However, it was a good upgrade, and the NX looks even better - Pre-programmed, easier to use, and with the USB feature. Pressure cookers and waterbaths generally give more consistent results than steamers since there is variation in the heating within a steam chamber so I think it will help you a lot to change over. With the new instruments' automation it should be painless to operate. Tim Morken UCSF Medical Center Pathology Dept -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Thursday, March 28, 2013 10:44 AM To: Swartwood, Steven J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Biocare Decloaker Nx Gen I would welcome input hear as well since we are looking at buying one as well. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Swartwood, Steven J Sent: Thursday, March 28, 2013 12:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare Decloaker Nx Gen Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope unmasking (heat retrieval)? Also does anyone have any personal pros/cons with this instrument? We are thinking about getting one because of inconsistencies with our current method using a vegetable steamer. We use many different tissue types both animal and human. Also we use many different antibodies. Currently with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure it wasn't due to fixation vimentin staining was performed and shows even staining. It's just certain antibodies that are giving us trouble even from multiple different companies. Any advice would be greatly appreciated and very beneficial to our work pertaining to the instrument. Thanks again everyone have a great end of the week, Steven Swartwood HT(ASCP) steven.swartw...@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Biocare Decloaker Nx Gen
We have used the Decloaking Chamber for several years now and have had excellent results. The nicest thing is that there is consistency and reliability. Each run will be the same as the one previous and having the ability to use the USB stick for documentation of time, pressure and temperature makes record keeping really easy. We have had no complaints. The only con I can come up with is that it comes in a big box that is hard to store if you have to send it back for some reason. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Swartwood, Steven J Sent: Thursday, March 28, 2013 1:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare Decloaker Nx Gen Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope unmasking (heat retrieval)? Also does anyone have any personal pros/cons with this instrument? We are thinking about getting one because of inconsistencies with our current method using a vegetable steamer. We use many different tissue types both animal and human. Also we use many different antibodies. Currently with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure it wasn't due to fixation vimentin staining was performed and shows even staining. It's just certain antibodies that are giving us trouble even from multiple different companies. Any advice would be greatly appreciated and very beneficial to our work pertaining to the instrument. Thanks again everyone have a great end of the week, Steven Swartwood HT(ASCP) steven.swartw...@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Biocare Decloaker Nx Gen
I have had the pleasure of seeing a demo of these Nxgen decloakers and I was very impressed. I currently use the older generation ones which still work very reliably for me. There is a nice tray inside the chamber to ensure the slide containers do not tip over and the USB which tracks all of the temperature and pressure information is a QC dream. The only thing which I could see as a down side would be the programming. It is preset from what I remember so you have to choose from the programs already existing. I think the existing programs should cover most antigens but if you wanted to be creative you don't have that option. I would love to have one in my lab ... Enjoy This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
