RE: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
Christina, The following might be useful: Kappel et al (HUM PATHOL 27:1361--1364, 1996) attempted to grow TB from formalin fixed lung tissue that had previously been shown to be positive by sputum culture. They were unable to culture TB from these tissues. Gerston et al (HUM PATHOL 35:571-575.2004) in South Africa analysed 138 formalin fixed lungs with histological evidence of AFB and were able to culture TB from 12 of these cases (one of these cases had been fixed for 80 days before being tested). Gerston et al suggest that there is a risk of contracting tuberculosis from tissue that has been fixed in formalin, if aerosols or accidental inoculation should occur. Trimming and sectioning wax blocks are of concern but no studies have been done yet. Of concern to histotechnolgists are: 1. Tissue with Inflammation-induced Encapsulation may protect bugs from formalin. 2. Formalin dilutes as it penetrates tissue. 3. Formalin substitutes may not be germicidal. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thurby, Christina Sent: Thursday, 14 April 2011 4:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? Hi, Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. Thanks, Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
Hi, Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. Thanks, Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
As long as possible. I remember reading somewhere about TB in lung tissue that was still viable after *years* of 10% NBF. Make sure the lungs are thoroughly perfused, or they will still be unfixed in the middle. There is a technique to inflate lungs at autopsy using a large syringe full of NBF and a clamp. Make sure that the specimen is not contacting the walls or bottom of the specimen container. You can suspend the whole organ with string inside the container, if needed. One can also use gauze or paper towels to keep the organ under the surface of the fixative. Lung tissue is notoriously difficult to fix properly, due both to the high lipid content of the pleural parenchyma, and the fact that fresh lungs float. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
5 days (96 h) is more than enough, but always handle the tissue and blocks using safety precautions. René J. --- On Wed, 4/13/11, Thurby, Christina christina.thu...@bms.com wrote: From: Thurby, Christina christina.thu...@bms.com Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, April 13, 2011, 2:04 PM Hi, Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. Thanks, Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
Hi, We fix 'Category-A' agent infected murine lungs in 10% buffered formalin for 7 days in ABSL-3. We also confirm tissue sterility by plating the tissue homogenates on suitable media before taking the samples out of ABSL-3 facility for sectioning. In your case 7 days should be enough, but it is always better to confirm the sterility of tissue. Thanks, Devender Devender Kumar, D.V.M., Ph.D. Postdoctorate fellow, Center for Immunology Microbial Disease, Albany Medical College, 47 New Scotland Avenue, MC 151 Albany, NY 12208 518.262.6220(LAB) -- -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 13, 2011 3:57 PM To: histonet@lists.utsouthwestern.edu; ChristinaThurby Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? 5 days (96 h) is more than enough, but always handle the tissue and blocks using safety precautions. René J. --- On Wed, 4/13/11, Thurby, Christina christina.thu...@bms.com wrote: From: Thurby, Christina christina.thu...@bms.com Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, April 13, 2011, 2:04 PM Hi, Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. Thanks, Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
