I have the bond and I use the BIO SB concentrate, RaBMab, EP190 at 1:50 and it
stains great on tonsil and kidney tubules.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: lseb...@uwhealth.org
> To: cda...@che-east.org; histonet@lists.utsouthwestern.edu
> Date: Thu, 13 Mar 2014 16:19:11 +
> CC:
> Subject: [Histonet] RE: MUM-1
>
> This is our protocol for the Ultra; maybe it will help.
>
> 64" CC1, 32" incubation (MUM-1, 760-4529) @ 36 degrees, Hem II/4". This is
> with UltraView DAB detection. We use tonsil as well however we validated
> with HD, LN, GI, tonsil, etc.
>
> Good luck!
>
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX: (608)262-7174
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie
> Sent: Thursday, March 13, 2014 8:54 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] MUM-1
>
> Good morning Histonet Folks,
>
> I am hoping one of you will help me. I am in the process of
> optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the
> Benchmark XTstainer and I am not thrilled with the results I am getting. I
> have tried the "usual adjustments" and the results are less than optimal in
> my opinion. I am using a normal tonsil control right now but if you have
> another suggestion please do not hesitate to recommend. I am praying somebody
> might have done this before and would be willing to share their staining
> protocol or tips with this.
>
> Cassandra Davis
> cda...@che-east.org
> 302-575-8095
>
>
>
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