Re: [Histonet] RE: Xylene Substitutes
I'm using Clear Rite 3 and I like it. Is there a better xylene substitute for animal tissues? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Xylene Substitutes
We use Propar, mostly for mouse and rat eye samples, it works well in our hands Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) [algra...@email.arizona.edu] Sent: Wednesday, August 21, 2013 9:47 AM Cc: HISTONET Subject: Re: [Histonet] RE: Xylene Substitutes I'm using Clear Rite 3 and I like it. Is there a better xylene substitute for animal tissues? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Xylene Substitutes
At another place I've worked for, we used Propar for animal tissues and it worked well. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, August 21, 2013 8:59 AM To: Grantham, Andrea L - (algranth) Cc: HISTONET Subject: RE: [Histonet] RE: Xylene Substitutes We use Propar, mostly for mouse and rat eye samples, it works well in our hands Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) [algra...@email.arizona.edu] Sent: Wednesday, August 21, 2013 9:47 AM Cc: HISTONET Subject: Re: [Histonet] RE: Xylene Substitutes I'm using Clear Rite 3 and I like it. Is there a better xylene substitute for animal tissues? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Xylene Substitutes
Adrienne Anderson (where?) asks: My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? I never heard of SubX, but the Leica Microsystems Richmond [no kin!] Inc. MSDS describes it as an Aliphatic hydrocarbon, isoparaffinic oil, of which there are a great number in the trade. The flash point is 106 degrees Fahrenheit (xylene is 78, some others as high as 104). These aliphatics are not compatible with each other if you're going to recover them by distillation, and with any of them you need to sit down with management and make clear that you're going to go with a particular trade name and that they can't substitute it with naphtha from a ma-and-pa repackaging operation. AmeriClear of course is limonene, a turpentine-like substance distilled from citrus peels. Many people find the citrus smell intolerable, or are allergic to it, and I think the limonenes (there are several other brands) are no longer in widespread use, particularly since the price has gone up. Please, folks, when you post trade names on HistoNet, take a moment to look at the MSDS (it's online) to see what the chemical identity of the trade-name product is. You should know this if you're using the product, and it's better to assume that other people don't know it. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Xylene substitutes
William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at least temporarily unavailable, and he is looking for a substitute. (Anybody know what's going on with Clear-Rite 3?) Clear-Rite 3 is an aliphatic (rather than a limonene). Aliphatics can't always be substituted for each other - they are chemically different from each other - each has its own flammability characteristics (flash point) and (if you're recovering solvent using a spinning band still) its own distillation routine. If you change, you may want to stay with the product you change to. Your purchasing people need to be aware that they are not to change brands on you. I just worked in a lab that has a Leica ASP300S processor (which they like, by the way). The people there have been told that they may use only one particular aliphatic with it, one I hadn't heard of before: Sub-X aliphatic hydrocarbon isoparaffinic oil CAS 6472-48-9DOT petroleum distillates, NOS, 3 class 3 flammable liquid Flash point 106 F (41 C) Anybody know if this is actually the case? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Xylene substitutes (UNCLASSIFIED)
Classification: UNCLASSIFIED Caveats: NONE I have 2 Leica ASP 300 tissue processors. I use Thermo Scientific Shandon Xylene Substitute (formally known as Histosolv). I have used this for many years with excellent results. I have never had any problems using this in the processor. We had 2 very old VIP tissue processors before we got the Leica processors and used Histosolv in those processors also. I have never heard of Sub-X. Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.glad...@amedd.army.mil Phone: 803-751- 2530 FAX: 803-751-7829 DSN: 734-2530 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, August 05, 2009 4:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Xylene substitutes William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at least temporarily unavailable, and he is looking for a substitute. (Anybody know what's going on with Clear-Rite 3?) Clear-Rite 3 is an aliphatic (rather than a limonene). Aliphatics can't always be substituted for each other - they are chemically different from each other - each has its own flammability characteristics (flash point) and (if you're recovering solvent using a spinning band still) its own distillation routine. If you change, you may want to stay with the product you change to. Your purchasing people need to be aware that they are not to change brands on you. I just worked in a lab that has a Leica ASP300S processor (which they like, by the way). The people there have been told that they may use only one particular aliphatic with it, one I hadn't heard of before: Sub-X aliphatic hydrocarbon isoparaffinic oil CAS 6472-48-9DOT petroleum distillates, NOS, 3 class 3 flammable liquid Flash point 106 F (41 C) Anybody know if this is actually the case? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
