[Histonet] RE: cleaning glassware
Also check the gelatine - is it a new batch? From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Anne Murvosh [amurv...@advancederm.net] Sent: Friday, 3 April 2015 6:01 AM To: Jeff Halstead; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: cleaning glassware I always used to rinse the containers I used with alcohol and let them dry before doing the stain. Some people use an acid alcohol rinse. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead Sent: Thursday, April 02, 2015 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning glassware Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cleaning glassware
I work in a small veterinary lab and when I do the Warthin Starry stain I mix all the reagents in never used before disposable plastic beakers and I stain the slides in an un-used slide mailer. Roberta Horner Animal Diagnostic Lab Penn State University -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead Sent: Thursday, April 02, 2015 2:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning glassware Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cleaning glassware
We always acid clean before doing any silver stain. No metal Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, April 02, 2015 2:36 PM To: Jeff Halstead; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: cleaning glassware I work in a small veterinary lab and when I do the Warthin Starry stain I mix all the reagents in never used before disposable plastic beakers and I stain the slides in an un-used slide mailer. Roberta Horner Animal Diagnostic Lab Penn State University -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead Sent: Thursday, April 02, 2015 2:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning glassware Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cleaning glassware
I always used to rinse the containers I used with alcohol and let them dry before doing the stain. Some people use an acid alcohol rinse. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead Sent: Thursday, April 02, 2015 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning glassware Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
