We have a medium GI lab using the old, old VIP's. We have been very successful
with two processing schedules, long and short. We found that we needed to add
a bit of heat to the last xylene so when the tissue hit the first paraffin,
they would start to infiltrate. The cold cassettes solidified the paraffin and
the time spent was melting the paraffin not infiltrating the biopsy. For the
longer run, we add 10%NBF and a bit longer in each solution.
Short:
1. 70% reagent alcohol - 15 min, 40C, P/V
2. 85% reagent alcohol - 10 min, 40C, P/V
3. 85% reagent alcohol - 15 min, 40C, P/V
4. 100% reagent alcohol - 10 min, 40C, P/V
5. 100% reagent alcohol - 10 min, 40C, P/V
6. 100% reagent alcohol - 10 min, 40C, P/V
7. Xylene - 20 min, 40C, P/V
8. Xylene - 20 min, 50C, P/V
9. Paraffin - 10 min, 58C, P/V
10. Paraffin - 30 min, 58C, P/V
11. Paraffin - 45 min, 58C, P/V
Karen Lahti, HT (ASCP), QIHC
Arizona Digestive Health
602-687-7468
On Apr 3, 2014, at 9:33 AM, Arguello, Daniel arguel...@ihn.org wrote:
I'd appreciate a copy of that processing schedule for GI bxs as well.
Thank you in advance
Sincerely,
Daniel Argüello, BS, CT (ASCP)CM
Anatomic Pathology Services Coordinator
Inspira Medical Center Woodbury
509 North Broad Street
Woodbury, NJ 08096
856-853-2030 Ext 2808
856-853-2183 Fax
arguel...@ihn.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vicki
McKaughan
Sent: Thursday, April 03, 2014 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] proceesing of GI Bxs
Could someone please send me your processing schedule for GI bxs. We are
using a Sakura VIP5 and are having problems with over drying and occasionally
with infiltration of paraffin. Thanks
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