[Histonet] RE: rolling sections
I agree - the sections don't need to roll. You can scrunch them and put them in the tube. I used to cut paraffin rolls / sections and manually extract DNA from them. You'll find that some roll very well some others not at all. My advice is to when you scrunch them, leave them more towards the bottom of the tube - often times when there's smaller pieces of wax in top part (and due to statics) upon opening the microtubes to start dewaxing there might be some pieces of wax coming out; same applies to smaller rolls. Also agree with the comment about increasing the thickness; however this might mean an extra step of xylene during the dewaxing process. And yes, do use and change gloves, blade and disinfect microtome sectioning area if the rolls are being used for nucleic acid extraction. Joana Joana Moreira Supervisor – Anatomical Pathology Department of Pathology Sidra Medical Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmore...@sidra.org | www.sidra.org -- Message: 7 Date: Thu, 12 Jun 2014 14:54:45 + From: Helen Fedor hfe...@jhmi.edu Subject: [Histonet] RE: rolling sections To: 'Roberta Horner' r...@psu.edu, Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Message-ID: bce07fb3a86fd040969a9ec0dd3eda0d170c7...@jhemtmwex4.win.ad.jhu.edu Content-Type: text/plain; charset=us-ascii Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 8 Date: Thu, 12 Jun 2014 14:58:03 + From: Martha Ward-Pathology mw...@wakehealth.edu Subject: [Histonet] RE: rolling sections To: Helen Fedor hfe...@jhmi.edu, 'Roberta Horner' r...@psu.edu, Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Message-ID: b2cecb1b6665a4479056478f6de3c4ab1985d...@exchdb6.medctr.ad.wfubmc.edu Content-Type: text/plain; charset=iso-8859-1 We do the same thing on our lab. It isn't necessary for them to rollwe just catch them and fold them up and put them in the tube. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mw...@wakehealth.edu ? ? ? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, June 12, 2014 10:55 AM To: 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 9 Date: Thu, 12 Jun 2014 14:58:46 + From: Bernice Frederick b-freder
[Histonet] RE: rolling sections
Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: rolling sections
That is how we do it also. Since they are not really interested in the sections being flat we just pick them and put them directly into the tube.If I am really having issues I will actually warm the block with my finger to help it roll. If they are doing DNA or RNAase ou may need to wear glove to prevent any contamination of the blocks. Pam Marcum UAMS - Original Message - From: Helen Fedor hfe...@jhmi.edu To: Roberta Horner r...@psu.edu, Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Sent: Thursday, June 12, 2014 9:54:45 AM Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: rolling sections
We do the same thing on our lab. It isn't necessary for them to rollwe just catch them and fold them up and put them in the tube. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, June 12, 2014 10:55 AM To: 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: rolling sections
True. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, June 12, 2014 9:58 AM To: Helen Fedor; 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: rolling sections We do the same thing on our lab. It isn't necessary for them to rollwe just catch them and fold them up and put them in the tube. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, June 12, 2014 10:55 AM To: 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: rolling sections
Hi Roberta, Check with those requesting to see if you can cut the equilivant but with thicker sections that will roll- like 5 20's or 2 50's. The rolls are lots easier to get into the tubes. Tom T -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Thursday, June 12, 2014 8:00 AM To: Helen Fedor Cc: Histonet (histonet@lists.utsouthwestern.edu); Roberta Horner Subject: Re: [Histonet] RE: rolling sections That is how we do it also. Since they are not really interested in the sections being flat we just pick them and put them directly into the tube.If I am really having issues I will actually warm the block with my finger to help it roll. If they are doing DNA or RNAase ou may need to wear glove to prevent any contamination of the blocks. Pam Marcum UAMS - Original Message - From: Helen Fedor hfe...@jhmi.edu To: Roberta Horner r...@psu.edu, Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Sent: Thursday, June 12, 2014 9:54:45 AM Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
