RE: [Histonet] Re: Flames at embedding centers
Darn right! We'll live forever at this rate of fixation... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, November 19, 2014 2:46 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Old Histotechs never die. They're just well fixed... :). From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Mark Turner [mtur...@csilaboratories.com] Sent: Wednesday, November 19, 2014 1:53 PM To: Sanders, Jeanine (CDC/OID/NCEZID); Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers We had a special clean counter we used for pizza on a regular basis. I worked with a pathologist who refused to wear gloves and would gross colons bare-handed. Guy is still alive and kicking at 85! In the very old days, we used carbon tetrachloride to dehydrate in the open tissue processor (Technicon). Not going to say anything at all about disposal Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:10 PM To: Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Okay, since we are confessing. When I was a student in histology school, we did the potluck thing IN THE LAB! I mean, ALL the food was laid out on a back counter -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, November 19, 2014 2:08 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Since the break room was right across the hall from the histology lab, we use to clear off a counter to put all the food for our potlucks. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:04 PM To: James Watson; 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers sigh the good old days -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, November 19, 2014 1:58 PM To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Flames at embedding centers
Ah, such an appropriate time of year for this thread. Once a year we gave our trash autoclave a good cleaning and pressure cooked turkeys for our Thanksgiving party. Ed Gray | Clinical Application Coordinator | Phone: 304-293-2945 | Fax: 304-293-1627 | WVU Healthcare l eg...@wvuhealthcare.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Flames at embedding centers
Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Flames at embedding centers
It would be interesting to create an album of memoirs to share with our younger generations. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Bob Richmond [rsrichm...@gmail.com] Sent: Wednesday, November 19, 2014 1:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Flames at embedding centers
How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Flames at embedding centers
sigh the good old days -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, November 19, 2014 1:58 PM To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Flames at embedding centers
Since the break room was right across the hall from the histology lab, we use to clear off a counter to put all the food for our potlucks. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:04 PM To: James Watson; 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers sigh the good old days -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, November 19, 2014 1:58 PM To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Flames at embedding centers
We had a special clean counter we used for pizza on a regular basis. I worked with a pathologist who refused to wear gloves and would gross colons bare-handed. Guy is still alive and kicking at 85! In the very old days, we used carbon tetrachloride to dehydrate in the open tissue processor (Technicon). Not going to say anything at all about disposal Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:10 PM To: Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Okay, since we are confessing. When I was a student in histology school, we did the potluck thing IN THE LAB! I mean, ALL the food was laid out on a back counter -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, November 19, 2014 2:08 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Since the break room was right across the hall from the histology lab, we use to clear off a counter to put all the food for our potlucks. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:04 PM To: James Watson; 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers sigh the good old days -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, November 19, 2014 1:58 PM To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Flames at embedding centers
Old Histotechs never die. They're just well fixed... :). From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Mark Turner [mtur...@csilaboratories.com] Sent: Wednesday, November 19, 2014 1:53 PM To: Sanders, Jeanine (CDC/OID/NCEZID); Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers We had a special clean counter we used for pizza on a regular basis. I worked with a pathologist who refused to wear gloves and would gross colons bare-handed. Guy is still alive and kicking at 85! In the very old days, we used carbon tetrachloride to dehydrate in the open tissue processor (Technicon). Not going to say anything at all about disposal Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:10 PM To: Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Okay, since we are confessing. When I was a student in histology school, we did the potluck thing IN THE LAB! I mean, ALL the food was laid out on a back counter -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, November 19, 2014 2:08 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Since the break room was right across the hall from the histology lab, we use to clear off a counter to put all the food for our potlucks. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:04 PM To: James Watson; 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers sigh the good old days -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, November 19, 2014 1:58 PM To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
