RE: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Perhaps all is not lost as you will be able to see on a H@E the spaces
reluctantly vacated by the lipids.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of z o n k e d
Sent: 13 November 2012 17:44
To: Jennifer MacDonald
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be
in OCT instead)!
We just wanted to see general lipids, nothing in particular. This mouse died
unexpectedly and may have been part of a group that was put on a high fat or
high bile acid diet and we just wanted to see what happened.
On Tuesday, November 13, 2012, Jennifer MacDonald wrote:
It depends on what you are using the oil red o for. Lipofuscin and
ceroid can be demonstrated with an oil red o stain after processing.
Jennifer MacDonald
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Date:11/13/2012 08:53 AM
Subject:[Histonet] Help! Liver mistakenly processed in paraffin
(had to bein OCT instead)!
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Hello Histonetters,
First time writer, long time reader. I'm a newbie tech in academia and
I was given a simple task which I think I pretty much screwed up.
I should have embedded half of a mouse liver in paraffin for microtome
sectioning while the other half should have been embedded in OCT for
cryosectioning (for oil red o). I made the mistake last night of
placing both liver halves into the tissue processor. The liver I
intended for OCT embedding is now hard as wax. Is there any way to
deparaffinize processed organs and may I embed them in OCT for proper
cryosectioning? I imagine that the liver would get dehydrated, I would
get crappy sections, and Oil Red O won't work.
Any suggestions are welcome.
Thank you so much,
Zoe W.
--
It costs nothing to say something kind. Even less to shut up altogether.
--Nathan Fillion
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--Nathan Fillion
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[Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Ah, I knew it. :( Thank you. Zoe On Tuesday, November 13, 2012, Will Chappell wrote: Nope, sorry. All your fat is dissolved. Sent from my iPhone On Nov 13, 2012, at 8:52 AM, z o n k e d zon...@gmail.com javascript:; wrote: Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu javascript:; http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
We just wanted to see general lipids, nothing in particular. This mouse
died unexpectedly and may have been part of a group that was put on a high
fat or high bile acid diet and we just wanted to see what happened.
On Tuesday, November 13, 2012, Jennifer MacDonald wrote:
It depends on what you are using the oil red o for. Lipofuscin and ceroid
can be demonstrated with an oil red o stain after processing.
Jennifer MacDonald
From:z o n k e d zon...@gmail.com javascript:_e({}, 'cvml',
'zon...@gmail.com');
To:histonet@lists.utsouthwestern.edu javascript:_e({}, 'cvml',
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'histonet@lists.utsouthwestern.edu');
Date:11/13/2012 08:53 AM
Subject:[Histonet] Help! Liver mistakenly processed in paraffin
(had to bein OCT instead)!
Sent by:histonet-boun...@lists.utsouthwestern.edujavascript:_e({},
'cvml', 'histonet-boun...@lists.utsouthwestern.edu');
--
Hello Histonetters,
First time writer, long time reader. I'm a newbie tech in academia and I
was given a simple task which I think I pretty much screwed up.
I should have embedded half of a mouse liver in paraffin for microtome
sectioning while the other half should have been embedded in OCT for
cryosectioning (for oil red o). I made the mistake last night of placing
both liver halves into the tissue processor. The liver I intended for OCT
embedding is now hard as wax. Is there any way to deparaffinize processed
organs and may I embed them in OCT for proper cryosectioning? I imagine
that the liver would get dehydrated, I would get crappy sections, and Oil
Red O won't work.
Any suggestions are welcome.
Thank you so much,
Zoe W.
--
It costs nothing to say something kind. Even less to shut up altogether.
--Nathan Fillion
___
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'Histonet@lists.utsouthwestern.edu');
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
--
It costs nothing to say something kind. Even less to shut up altogether.
--Nathan Fillion
___
Histonet mailing list
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[Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
if there was fat replacement, such as cirrhosis, you will see it in the morphology. From: z o n k e d zon...@gmail.com To: Jennifer MacDonald jmacdon...@mtsac.edu Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu Date: 11/13/2012 09:43 AM Subject:Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened. On Tuesday, November 13, 2012, Jennifer MacDonald wrote: It depends on what you are using the oil red o for. Lipofuscin and ceroid can be demonstrated with an oil red o stain after processing. Jennifer MacDonald From:z o n k e d zon...@gmail.com To:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date:11/13/2012 08:53 AM Subject:[Histonet] Help! Liver mistakenly processed in paraffin (had to bein OCT instead)! Sent by:histonet-boun...@lists.utsouthwestern.edu Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Thank you all for your helpful responses! I will go ahead and carry on with paraffin sectioning and stain with HE as planned. As for the Oil Red O, I'll try that too and see what happens. (Still amazed at how amazing Histonet is and how helpful you all are! Thank you very much for all your responses!) On Tue, Nov 13, 2012 at 2:01 PM, Jennifer MacDonald jmacdon...@mtsac.eduwrote: if there was fat replacement, such as cirrhosis, you will see it in the morphology. From:z o n k e d zon...@gmail.com To:Jennifer MacDonald jmacdon...@mtsac.edu Cc:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu Date:11/13/2012 09:43 AM Subject:Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! -- We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened. On Tuesday, November 13, 2012, Jennifer MacDonald wrote: It depends on what you are using the oil red o for. Lipofuscin and ceroid can be demonstrated with an oil red o stain after processing. Jennifer MacDonald From:z o n k e d *zon...@gmail.com* To:*histonet@lists.utsouthwestern.edu* * histonet@lists.utsouthwestern.edu* Date:11/13/2012 08:53 AM Subject:[Histonet] Help! Liver mistakenly processed in paraffin (had to bein OCT instead)! Sent by:*histonet-boun...@lists.utsouthwestern.edu* -- Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list* **Histonet@lists.utsouthwestern.edu** **http://lists.utsouthwestern.edu/mailman/listinfo/histonet*http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
