RE: [Histonet] Re: Negative Controls
So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: must show no staining of tissues known to lack the antigen Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered best practice... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual. Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar bbrinegar...@gmail.com Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Negative Controls
As of now, my lab took that as you just needed one negative slide per run. And that negative slide is getting the negative mouse or rabbit serum. Is this correct? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: must show no staining of tissues known to lack the antigen Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered best practice... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual. Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar bbrinegar...@gmail.com Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Negative Controls
Tasha, We use the negative elements within our patient sample as our negative tissue control. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, April 30, 2014 6:48 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: must show no staining of tissues known to lack the antigen Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered best practice... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual. Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar bbrinegar...@gmail.com Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Negative Controls
So do we. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, April 30, 2014 8:35 AM To: 'Campbell, Tasha M.'; Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls Tasha, We use the negative elements within our patient sample as our negative tissue control. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, April 30, 2014 6:48 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: must show no staining of tissues known to lack the antigen Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered best practice... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual. Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar bbrinegar...@gmail.com Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Negative Controls
So do we Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, April 30, 2014 8:35 AM To: 'Campbell, Tasha M.'; Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls Tasha, We use the negative elements within our patient sample as our negative tissue control. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, April 30, 2014 6:48 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: must show no staining of tissues known to lack the antigen Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered best practice... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual. Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar bbrinegar...@gmail.com Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including
[Histonet] Re: Negative Controls
On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: must show no staining of tissues known to lack the antigen Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered best practice... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual. Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar bbrinegar...@gmail.com Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Negative Controls for IHC
I apologize for the delay in responding to your e-mail; I just returned from the 2014 Applied IHC and Molecular Morphology meeting in Miami where this subject was discussed (as you might expect). The answer is, Yes. The bottom line is that every laboratory running non-avidin-biotin-based diagnostic IHC tests must evaluate their own experience with Negative Reagent Controls (NRCs). If they prove useful to the pathologists reading the IHC slides, continue to run them. If not, discontinue them and conserve precious patient tissue/cells that can be used for additional testing, and, at the same time, save you laboratory thousands of dollars. I have an Editorial on this subject coming out in a major journal next month. I've been told that there is an article coming as well. I added NRC to our antibody dictionary years ago so that pathologists can order a NRC whenever they need it after reviewing their IHC slides. During a 5 year period, only 28 NRCs were ordered and I ordered 22 of them (primarily for education and teaching purposes). Keep in mind that my laboratory runs 40-50,000 IHC tests per year. Make you own decision Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, February 11, 2014 1:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Controls for IHC I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Negative Controls for IHC
Cheryl Yes, I understand. It is highly likely this will be the way things go here also. Joelle Weaver MAOM, HTL (ASCP) QIHC From: cmil...@physlab.com To: joellewea...@hotmail.com CC: histonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Date: Wed, 12 Feb 2014 08:04:43 -0600 Subject: RE: [Histonet] RE: Negative Controls for IHC I just stopped using the reagent controls on all our IHC due to the drastic cuts in reimbursement. It will be a significant immediate savings for our tiny lab. Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver [joellewea...@hotmail.com] Sent: Tuesday, February 11, 2014 1:54 PM To: Sebree Linda A; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Negative Controls for IHC Not too long ago, there was a posting about labs that had discontinued. You technically don't need with a polymer, non-biotin system. I still do them- for now. The pathologist never uses them, internal controls only. But I do, but this may be a good cost cutting idea for the future. Joelle Weaver MAOM, HTL (ASCP) QIHC From: lseb...@uwhealth.org To: tanyaabb...@catholichealth.net; histonet@lists.utsouthwestern.edu Date: Tue, 11 Feb 2014 19:44:37 + CC: Subject: [Histonet] RE: Negative Controls for IHC Hi Tanya, We have made the decision to continue running negative reagent control slides. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, February 11, 2014 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Controls for IHC I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Negative Controls for IHC
We discontinued them over a year ago. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, February 12, 2014 11:04 AM To: Cheri Miller Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Negative Controls for IHC Cheryl Yes, I understand. It is highly likely this will be the way things go here also. Joelle Weaver MAOM, HTL (ASCP) QIHC From: cmil...@physlab.com To: joellewea...@hotmail.com CC: histonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Date: Wed, 12 Feb 2014 08:04:43 -0600 Subject: RE: [Histonet] RE: Negative Controls for IHC I just stopped using the reagent controls on all our IHC due to the drastic cuts in reimbursement. It will be a significant immediate savings for our tiny lab. Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver [joellewea...@hotmail.com] Sent: Tuesday, February 11, 2014 1:54 PM To: Sebree Linda A; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Negative Controls for IHC Not too long ago, there was a posting about labs that had discontinued. You technically don't need with a polymer, non-biotin system. I still do them- for now. The pathologist never uses them, internal controls only. But I do, but this may be a good cost cutting idea for the future. Joelle Weaver MAOM, HTL (ASCP) QIHC From: lseb...@uwhealth.org To: tanyaabb...@catholichealth.net; histonet@lists.utsouthwestern.edu Date: Tue, 11 Feb 2014 19:44:37 + CC: Subject: [Histonet] RE: Negative Controls for IHC Hi Tanya, We have made the decision to continue running negative reagent control slides. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, February 11, 2014 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Controls for IHC I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http
[Histonet] RE: Negative Controls for IHC
Hi Tanya, We have made the decision to continue running negative reagent control slides. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, February 11, 2014 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Controls for IHC I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Negative Controls for IHC
Not too long ago, there was a posting about labs that had discontinued. You technically don't need with a polymer, non-biotin system. I still do them- for now. The pathologist never uses them, internal controls only. But I do, but this may be a good cost cutting idea for the future. Joelle Weaver MAOM, HTL (ASCP) QIHC From: lseb...@uwhealth.org To: tanyaabb...@catholichealth.net; histonet@lists.utsouthwestern.edu Date: Tue, 11 Feb 2014 19:44:37 + CC: Subject: [Histonet] RE: Negative Controls for IHC Hi Tanya, We have made the decision to continue running negative reagent control slides. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, February 11, 2014 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Controls for IHC I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Negative Controls
The Evidence should come from your own laboratory. Just because we don't run Negative reagent controls in my laboratory doesn't mean it's appropriate for you to discontinue their use for your laboratory. Your laboratory Medical Director needs to make this decision based on what he/she sees (or doesn't see) on your Negative reagent controls. In my experience with polymer detection we rarely see nonspecific staining due to the detection reagents on our Negative reagent controls. As I have stated before, I have given my pathologist colleagues the option of ordering a Negative reagent control if they feel they need it (after reviewing the original immunoperoxidase stains) and, in over 5 years, I have only seen a handful of orders (mostly for tissues that have endogenous pigment). If you are seeing a lot of nonspecific reactivity on your Negative reagent controls that complicates interpretation then you should run them. I don't; therefore, I am preserving precious tissue for important testing and saving our institution thousands of dollars each year. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax Ian R Bernard ibern...@uab.edu 10/4/2012 9:28 AM This is the key. What is the evidence based research/studies to support this? I will continue with negative controls until I am able to get peer reviewed research to support this. Besides overkill maybe pricey ($$) at times or not (since it is just slides) but from what I learned this past week at NSH 2012 Vancouver workshop: There are other important costs for us to consider thus we need to count the cost of poor quality. We learned that there are three types of other costs to consider in the medical laboratory: prevention, appraisal and failure (internal and external) costs. According to our workshop presenter from Vancouver, his or the research shows that failure costs are a whole lot more than the cost of prevention and appraisal. Therefore a focus on things like time, patients, reputation and staff costs is necessary as well as money. IB -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Friday, August 17, 2012 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Controls Does anyone know of any articles talking about not using negative controls when using a polymer based detection system. Judith Pardue Memorial Hospital Chattanooga, Tn. judith_par...@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Negative Controls
This is the key. What is the evidence based research/studies to support this? I will continue with negative controls until I am able to get peer reviewed research to support this. Besides overkill maybe pricey ($$) at times or not (since it is just slides) but from what I learned this past week at NSH 2012 Vancouver workshop: There are other important costs for us to consider thus we need to count the cost of poor quality. We learned that there are three types of other costs to consider in the medical laboratory: prevention, appraisal and failure (internal and external) costs. According to our workshop presenter from Vancouver, his or the research shows that failure costs are a whole lot more than the cost of prevention and appraisal. Therefore a focus on things like time, patients, reputation and staff costs is necessary as well as money. IB -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Friday, August 17, 2012 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Controls Does anyone know of any articles talking about not using negative controls when using a polymer based detection system. Judith Pardue Memorial Hospital Chattanooga, Tn. judith_par...@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: negative controls
For everyone performing immunos are you not running negative controls now with this new ruling from CAP? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Romundstad, Pamela K Sent: Friday, August 17, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 105, Issue 20 Yes, the negative control updated change is in effect as of July 31. 2012. I contacted CAP personally this week and was directed to the revised ANP.22570. It only applies to polymer based detection systems (biotin-free). Respectfully, Pamela Romundstad HT, QIHC Gundersen Lutheran 608-775-3139 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Thursday, August 16, 2012 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. protocol for EGFP IHC in frozen rat brains (Bass, Caroline) 2. Re: Two identifiers... (Bob Richmond) 3. RE: Re: Two identifiers... (joelle weaver) 4. RE: Re: Two identifiers... (Michael Mihalik) 5. Negative Control (Pardue, Judith) 6. RE: Special Stain documentation (hymclab) 7. Peloris (Pardue, Judith) 8. RE: RE: Two identifiers... (Pratt, Caroline) 9. RE: RE: Two identifiers... (Tom McNemar) 10. marker specific for mouse fibroblasts (Kim Merriam) 11. RE: RE: Two identifiers... (Pratt, Caroline) 12. RE: marker specific for mouse fibroblasts (Elizabeth Chlipala) -- Message: 1 Date: Wed, 15 Aug 2012 18:07:46 -0400 From: Bass, Caroline ceb...@buffalo.edu Subject: [Histonet] protocol for EGFP IHC in frozen rat brains To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: abc535ec-9fcd-45e0-957c-617fa7aa8...@buffalo.edu Content-Type: text/plain; charset=Windows-1252 Hello Everyone, I have some rat brains that should express GFP, the brains were collected fresh and snap frozen in dry ice/isopentane. They have been stored for several months in a -80. I'd like to collect 500 um sections, thaw mount them to a slide an reserve these for mRNA collection. I would then like to take intervening sections to stain for GFP. I have a staining protocol for perfused, formalin fixed, sucrose protected floating brain sections that works fine. I'm a little more concerned about moving to unfixed sections. I know I need to cut everything on a cryostat and I assume that I have to process the thinner sections on the slide instead of free floating. What is the best way to proceed? Here are some specific questions? 1) what thickness should I cut, 50 um works great for my fixed sections. 2) should I fix these sections by either paraformaldehyde vapors or dunking in fix? If so, what kind/concentration? 3) could I then proceed as though this were a normal GFP immuno, with DAB stain? 4) should I used plus slides or subbed slides? 5) will the sections stay on without fixation? Any and all suggestions would be appreciated! Thanks, Caroline -- Message: 2 Date: Wed, 15 Aug 2012 21:11:12 -0400 From: Bob Richmond rsrichm...@gmail.com Subject: [Histonet] Re: Two identifiers... To: histonet@lists.utsouthwestern.edu Message-ID: caoksrh5bhasnonck7zg+04qjd23o0nfo+5t-8fjl71hs6af...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 If a pathologist end-user may make an observation here: When I pick up a slide and look to match it with the paperwork in front of me, it's a great help if the patient's name is on the slide, since names are always easier to read than numbers. Having the patient's name on the slide means I'm less likely to mix up two cases. I can't do that with a bar code or a second essentially meaningless number. This may however be a situation where good patient care must take a back seat to good management practice and good regulator
[Histonet] RE: negative controls
We aren't. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Friday, August 17, 2012 12:36 PM To: 'Romundstad, Pamela K'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: negative controls For everyone performing immunos are you not running negative controls now with this new ruling from CAP? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Romundstad, Pamela K Sent: Friday, August 17, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 105, Issue 20 Yes, the negative control updated change is in effect as of July 31. 2012. I contacted CAP personally this week and was directed to the revised ANP.22570. It only applies to polymer based detection systems (biotin-free). Respectfully, Pamela Romundstad HT, QIHC Gundersen Lutheran 608-775-3139 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Thursday, August 16, 2012 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. protocol for EGFP IHC in frozen rat brains (Bass, Caroline) 2. Re: Two identifiers... (Bob Richmond) 3. RE: Re: Two identifiers... (joelle weaver) 4. RE: Re: Two identifiers... (Michael Mihalik) 5. Negative Control (Pardue, Judith) 6. RE: Special Stain documentation (hymclab) 7. Peloris (Pardue, Judith) 8. RE: RE: Two identifiers... (Pratt, Caroline) 9. RE: RE: Two identifiers... (Tom McNemar) 10. marker specific for mouse fibroblasts (Kim Merriam) 11. RE: RE: Two identifiers... (Pratt, Caroline) 12. RE: marker specific for mouse fibroblasts (Elizabeth Chlipala) -- Message: 1 Date: Wed, 15 Aug 2012 18:07:46 -0400 From: Bass, Caroline ceb...@buffalo.edu Subject: [Histonet] protocol for EGFP IHC in frozen rat brains To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: abc535ec-9fcd-45e0-957c-617fa7aa8...@buffalo.edu Content-Type: text/plain; charset=Windows-1252 Hello Everyone, I have some rat brains that should express GFP, the brains were collected fresh and snap frozen in dry ice/isopentane. They have been stored for several months in a -80. I'd like to collect 500 um sections, thaw mount them to a slide an reserve these for mRNA collection. I would then like to take intervening sections to stain for GFP. I have a staining protocol for perfused, formalin fixed, sucrose protected floating brain sections that works fine. I'm a little more concerned about moving to unfixed sections. I know I need to cut everything on a cryostat and I assume that I have to process the thinner sections on the slide instead of free floating. What is the best way to proceed? Here are some specific questions? 1) what thickness should I cut, 50 um works great for my fixed sections. 2) should I fix these sections by either paraformaldehyde vapors or dunking in fix? If so, what kind/concentration? 3) could I then proceed as though this were a normal GFP immuno, with DAB stain? 4) should I used plus slides or subbed slides? 5) will the sections stay on without fixation? Any and all suggestions
[Histonet] RE: negative controls
We eliminated them on August 1st, except in cases where they are specifically requested. So far we have run 10 negative slides. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Friday, August 17, 2012 12:36 PM To: 'Romundstad, Pamela K'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: negative controls For everyone performing immunos are you not running negative controls now with this new ruling from CAP? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Romundstad, Pamela K Sent: Friday, August 17, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 105, Issue 20 Yes, the negative control updated change is in effect as of July 31. 2012. I contacted CAP personally this week and was directed to the revised ANP.22570. It only applies to polymer based detection systems (biotin-free). Respectfully, Pamela Romundstad HT, QIHC Gundersen Lutheran 608-775-3139 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Thursday, August 16, 2012 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. protocol for EGFP IHC in frozen rat brains (Bass, Caroline) 2. Re: Two identifiers... (Bob Richmond) 3. RE: Re: Two identifiers... (joelle weaver) 4. RE: Re: Two identifiers... (Michael Mihalik) 5. Negative Control (Pardue, Judith) 6. RE: Special Stain documentation (hymclab) 7. Peloris (Pardue, Judith) 8. RE: RE: Two identifiers... (Pratt, Caroline) 9. RE: RE: Two identifiers... (Tom McNemar) 10. marker specific for mouse fibroblasts (Kim Merriam) 11. RE: RE: Two identifiers... (Pratt, Caroline) 12. RE: marker specific for mouse fibroblasts (Elizabeth Chlipala) -- Message: 1 Date: Wed, 15 Aug 2012 18:07:46 -0400 From: Bass, Caroline ceb...@buffalo.edu Subject: [Histonet] protocol for EGFP IHC in frozen rat brains To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: abc535ec-9fcd-45e0-957c-617fa7aa8...@buffalo.edu Content-Type: text/plain; charset=Windows-1252 Hello Everyone, I have some rat brains that should express GFP, the brains were collected fresh and snap frozen in dry ice/isopentane. They have been stored for several months in a -80. I'd like to collect 500 um sections, thaw mount them to a slide an reserve these for mRNA collection. I would then like to take intervening sections to stain for GFP. I have a staining protocol for perfused, formalin fixed, sucrose protected floating brain sections that works fine. I'm a little more concerned about moving to unfixed sections. I know I need to cut everything on a cryostat and I assume that I have to process the thinner sections on the slide instead of free floating. What is the best way to proceed? Here are some specific questions? 1) what thickness should I cut, 50 um works great for my fixed sections. 2) should I fix these sections by either paraformaldehyde vapors or dunking in fix? If so, what kind/concentration? 3) could I then proceed as though this were a normal GFP immuno, with DAB stain? 4) should I used plus slides or subbed slides? 5) will the sections stay on without fixation? Any and all suggestions would be appreciated! Thanks, Caroline -- Message: 2 Date: Wed, 15 Aug 2012 21:11:12 -0400 From: Bob Richmond rsrichm...@gmail.com Subject: [Histonet] Re: Two identifiers... To: histonet@lists.utsouthwestern.edu Message-ID: caoksrh5bhasnonck7zg+04qjd23o0nfo+5t
[Histonet] RE: negative controls on immunos
Yes, you should run negative controls with each run - today and tomorrow. Something can happen in tomorrow's run so tomorrow's negative control will have different issues from today's negative control. The negative control should go through everything that your slides go through, whether it's today's great run or tomorrows electrical black out or whatever mishap. Whenever you run a test you must have a positive control and a negative control. Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ 07103 USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Tuesday, March 01, 2011 1:30 PM To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: negative controls on immunos We do not run additional negatives with each run on the same block. If it was a different block then yes we would run a negative. It takes up too many spaces on the machine and uses reagents. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig [dmcc...@ckha.on.ca] Sent: Tuesday, March 01, 2011 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls on immunos If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: negative controls on immunos
I was also taught to run a positive and negative every time you do an immuno stain, provided there is sufficient tissue. I was trained at AFIP, by the people that wrote the second AFIP staining manual (the red one). Of course, if cost is your number one concern. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
