Sadly, it is much safer than the old Chloroform clearing method.
Claire
From: Tony Henwood (SCHN) via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Sunday, March 26, 2017 6:22 PM
To: ian bernard
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Reference for this Recipe and Use
This is sometimes known as Newel's solution for revealing lymph nodes in fatty
specimens:
Newell KJ, Sawka BW, Rudrick BF, Driman DK. (2001) "GEWF solution" Arch Pathol
Lab Med 125:642 645.
Absolute ethanol1000ml
Water 340ml
Concentrated formalin (37%) 160ml
Glacial acetic acid 100ml
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-Original Message-
From: ian bernard via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Saturday, 25 March 2017 3:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Reference for this Recipe and Use
500 ML OF 100% ALCOHOL
170 ML OF DISTILLED WATER
80 ML OF 40% FORMALIN
50 ML OF GLACIAL ACETIC ACID
V/R
IB
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