Re: [Histonet] Research microtomes
This post is actually on behalf of some interns that are working at my company over the summer. Their emails are included on this post, so I'm going to turn it over to them:) But here is their description of what they're doing: In answer to Jack's questions (I thought about replying myself, but didn't want to confuse anyone), we want to make a CEMA array (cutting edge matrix assembly array), which could be done with a variety of tissue types. The step that calls for the special microtome is when you cut the original blocks from the hospital -- meaning variable tissue depth. Though the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns. Thanks, Adrienne From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 6:13 PM Subject: Re: [Histonet] Research microtomes Couple more questions. :) What is the tissue and the dimensions of the specimen? On Jul 20, 2011, at 4:57 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hi Jack, We're trying to cut just plain old FFPE blocks. From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 5:02 PM Subject: Re: [Histonet] Research microtomes What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hello Histo-land, I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! Thanks, Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Research microtomes
Thanks a lot for your response Adrienne! I think I may be able to help you with this. Is there a time we can talk off-line over the phone? My number is 317-281-1975. Best Regards, Jack On Jul 21, 2011, at 9:49 AM, Adrienne Anderson rennie1...@yahoo.com wrote: This post is actually on behalf of some interns that are working at my company over the summer. Their emails are included on this post, so I'm going to turn it over to them:) But here is their description of what they're doing: In answer to Jack's questions (I thought about replying myself, but didn't want to confuse anyone), we want to make a CEMA array (cutting edge matrix assembly array), which could be done with a variety of tissue types. The step that calls for the special microtome is when you cut the original blocks from the hospital -- meaning variable tissue depth. Though the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns. Thanks, Adrienne From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 6:13 PM Subject: Re: [Histonet] Research microtomes Couple more questions. :) What is the tissue and the dimensions of the specimen? On Jul 20, 2011, at 4:57 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hi Jack, We're trying to cut just plain old FFPE blocks. From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 5:02 PM Subject: Re: [Histonet] Research microtomes What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hello Histo-land, I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! Thanks, Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Research microtomes
Hi Jack, We're trying to cut just plain old FFPE blocks. From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 5:02 PM Subject: Re: [Histonet] Research microtomes What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hello Histo-land, I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! Thanks, Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Research microtomes
Couple more questions. :) What is the tissue and the dimensions of the specimen? On Jul 20, 2011, at 4:57 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hi Jack, We're trying to cut just plain old FFPE blocks. From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 5:02 PM Subject: Re: [Histonet] Research microtomes What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hello Histo-land, I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! Thanks, Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
