Valerie,
I use just regular glass slides, coat them with Haupt's adhesive, roll the
sections flat and then press and dry the sections to the coated slide using a
slide press and oven at 50C. In 13 years of hard tissue resin histology, I have
never (knocking twice on wood) lost a section or had a section lift up in any
area during staining using this method. You will get a degree of background
with certain stains (hematoxylin, analine blue, etc) because it is a gelatin
based adhesive, but these problems are easily resolved with a little
overstaining and acid alcohol rinse.
If interested you can get the Haupt's and slide press from Dorn and Hart
Microedge (www.dornandhart.com). I have also been told that they have a couple
of new kits coming out by the end of the summer - a resin embedding kit (using
Perkadox as a catalyst) and thin section microtomy kit (one for a rotary
microtome and one for a sledge or Polycut microtome) that includes everything
needed to section resin blocks. Look them up and contact Bill Hart for more
information.
Jack
On Jul 15, 2010, at 3:09 AM, Tilston, Valerie v.tils...@liverpool.ac.uk
wrote:
Hi,
Does anyone have experience of cutting resin embedded bone and if so what
type of slides do people use? We are having problems with sections falling
off or the bone not remaining flat on the slide!
Many thanks in advance,
Val
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