Re: [Histonet] Retic staining

[Debra Siena via Histonet]

Hi all

Also the freshness of the ammonium hydroxide can make a difference in how much 
you have to add to get the proper reactions. Fresher works better and faster. 
Also I use one of the plastic disposable properties to do drop by drop while 
swirling in s small acid cleaned flask or new plastic container.

Debbie Siena

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From: Cassie P. Davis via Histonet 
Sent: Tuesday, April 23, 2019 1:07 PM
To: histonet
Subject: [Histonet] Retic staining


*** Externally sourced email message ***



Hi Charles,


in addition to the drop size clean glassware makes a big difference. I used to 
use new urine cups to ensure the silver was being made in a clean vessel. I 
hope this helps


Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email: cda...@che-east.org<mailto:cda...@che-east.org>



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Re: [Histonet] Retic staining

[deGuzman, Jose R via Histonet]

Hi Charles,

Ammoniacal Silver is a tricky step, we've had a lot of repeat Bielschowsky 
stains in the past due to it. You don't need to go drop by drop, what's key is 
to gently stir the solution as you slowly add ammonium hydroxide until the 
precipitate is reincorporated back into the solution. I don't know if your 
protocol has a specific amount of ammonium hydroxide to add but we've found 
that sometimes it takes less and sometimes it takes more than what is 
recommended to clear. Make sure you stir and keep an eye on the solution to 
make sure you only add what is needed for the reaction.

Jose



-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: Tuesday, April 23, 2019 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 185, Issue 14

I am having trouble getting my retic stain to come out strong. It is working 
but Pathologists are saying they shouldn't have to go to high power just to see 
it (and I agree).

I am using a brand new American MasterTech Chandler's Precision kit. I have 
followed all the steps correctly (one difficulty is getting the Ammonium 
hydroxide to go drop by drop so this may be the issue).

I have tried staining longer in ammoniacal silver. more time in 20% formalin,  
more and less time in gold chloride and sodium thiosulfate.

If anyone has any suggestions or uses this kit with time modifications please 
let me know. Willing to try anything to get it working.


-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs

--
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[Histonet] Retic staining

[Cassie P. Davis via Histonet]

Hi Charles,


in addition to the drop size clean glassware makes a big difference. I used 
to use new urine cups to ensure the silver was being made in a clean vessel. I 
hope this helps


Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email:  cda...@che-east.org



Confidentiality Notice:
This e-mail, including any attachments is the property of Trinity Health and is 
intended for the sole use of the intended recipient(s). It may contain 
information that is privileged and confidential.  Any unauthorized review, use, 
disclosure, or distribution is prohibited. If you are not the intended 
recipient, please delete this message, and reply to the sender regarding the 
error in a separate email.
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[Histonet] Retic staining

[Charles Riley via Histonet]

I am having trouble getting my retic stain to come out strong. It is
working but Pathologists are saying they shouldn't have to go to high power
just to see it (and I agree).

I am using a brand new American MasterTech Chandler's Precision kit. I have
followed all the steps correctly (one difficulty is getting the Ammonium
hydroxide to go drop by drop so this may be the issue).

I have tried staining longer in ammoniacal silver. more time in 20%
formalin,  more and less time in gold chloride and sodium thiosulfate.

If anyone has any suggestions or uses this kit with time modifications
please let me know. Willing to try anything to get it working.


-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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