[Histonet] S100
Our S100 is not working very well. I think it's too old. Can someone recommend an S100 antibody that works well in dog? It would be really nice to find a good monoclonal instead of the polyclonals. We are looking for schwannomas, astrogliomas, malignant melanomas and their metastases. Margaret Perry HT(ASCP) Veterinary Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] S100
Margaret, What S100 are you using? We use Dako's polyclonal, no antigen retrieval needed, with no problems. Jan Shivers UMN VDL On Wed, May 8, 2013 at 2:25 PM, Perry, Margaret margaret.pe...@sdstate.eduwrote: Our S100 is not working very well. I think it's too old. Can someone recommend an S100 antibody that works well in dog? It would be really nice to find a good monoclonal instead of the polyclonals. We are looking for schwannomas, astrogliomas, malignant melanomas and their metastases. Margaret Perry HT(ASCP) Veterinary Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] S100, NEUROFILAMENT, AND CD10
I would like to know what protocols people are using for S100 (m) from Ventana and Neurofilament from Ventana and CD10 from Ventana on the Bench XT using ultaview DAB. Thanks, Rachel. This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] S100 primary antibody suitable for frozen sections?
I would appreciate hearing from anyone who has discovered an S100 primary antibody that is confirmed to work on frozen sections using a conventional IHC stain protocol. This will be utilized in a busy Mohs Surgery laboratory where staining time must be kept as brief as possible. The commercially available antibodies I've seen for this antigen are intended for FFPE tissues and initial attempts to adapt them to frozen sections have been unsuccessful. our surgeons prefer to avoid post fixation prior to staining although I'm willing to re-visit this if it is my only option. Thank you everyone. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] S100 A1
I'm looking for a mouse anti human S100 A1 antibody for IHC. My vendor doesn't carry it anymore. Anyone have one that they are using on FFPE tissue? Could you send me ordering info or a copy of your data sheet? Thanks, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
