[Histonet] SRBS/van Gieson

2013-01-18 Thread Herrick, James L. (Jim) 
Happy Friday Everybody,

We are trying to develop a protocol for an SRBS/van Gieson's stain on MMA 
embedded tissue. The sections are 5um in thickness and deplasticized. Our 
protocol seems to work ok on thick sections but not on thin, deplasticized 
sections. Any thoughts or ideas would be greatly appreciated. Hope you all have 
a great weekend!!

Thanks,
Jim


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Re: [Histonet] SRBS/van Gieson

2013-01-18 Thread Jack Ratliff 
What is it that you specifically want to accomplish that you can't do with a 
VonKossa/MacNeals Tetrachrome or Goldner's Trichrome Stain?

Jack


On Jan 18, 2013, at 1:51 PM, Herrick, James L. (Jim) herrick.ja...@mayo.edu 
wrote:

 Happy Friday Everybody,
 
 We are trying to develop a protocol for an SRBS/van Gieson's stain on MMA 
 embedded tissue. The sections are 5um in thickness and deplasticized. Our 
 protocol seems to work ok on thick sections but not on thin, deplasticized 
 sections. Any thoughts or ideas would be greatly appreciated. Hope you all 
 have a great weekend!!
 
 Thanks,
 Jim
 
 
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 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 

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