Re: [Histonet] Sanderson's rapid bone stain + Acid fuchsin
Cathy Sanderson wrote a review article about staining undecalcified bone only 11 years ago (which is recent in the literature of histological techniques): Sanderson, C. (1997) Entering the realm of mineralized bone processing: A review of the literature and techniques. J. Histotechnol. 20:259-266. If you're a member of the NSH you can request PDF files from the journal by way of the society's web site. (I hope this is still so; I haven't requested one in the last year.) There is an excellent chapter on bonology by Gayle Callis in Bancroft, J.D., and Gamble, M. (eds). Theory and Practice of Histological Techniques: London: Churchill Livingstone. It's Chapter 14 in the 5th edition (2002). I don't have the 6th (2008) edition; chapter numbers may not be the same. For discussion of the principles involved in staining sections of plastic-embedded tissue, see: Horobin RW (1982) Histochemistry: An Explanatory Outline of Histochemistry and Biophysical Staining. Gustav Fischer, Stuttgart. Horobin RW (1988) Understanding Histochemistry: Selection, Evaluation and Design of Biological Stains. Ellis Horwood, Chichester. Acid fuchsine and basic fuchsine have the same colour but almost opposite staining properties, which have been quite well understood since about 1880. John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: sk...@illinois.edu Date: Monday, December 21, 2009 14:26 Subject: [Histonet] Sanderson's rapid bone stain + Acid fuchsin To: histonet@lists.utsouthwestern.edu Hi All - I am trying to figure out if the combination of Sanderson's rapid bone stain and acid fuchsin as the counterstain (or methylene blue + basic fuchsin) results in pink/red staining of biologically formed apatite (apatite not associated with collagen produced by osteoblasts)? An associated question is if the dye-tissue interaction between acid or basic fuchsin and mineralized bone is due to dye-collagen interactions? If anyone has any experiences or references that may help, please let me know. Thank you for your help, Sheeny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sanderson's rapid bone stain + Acid fuchsin
Hi All - I am trying to figure out if the combination of Sanderson's rapid bone stain and acid fuchsin as the counterstain (or methylene blue + basic fuchsin) results in pink/red staining of biologically formed apatite (apatite not associated with collagen produced by osteoblasts)? An associated question is if the dye-tissue interaction between acid or basic fuchsin and mineralized bone is due to dye-collagen interactions? If anyone has any experiences or references that may help, please let me know. Thank you for your help, Sheeny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sanderson's rapid bone stain + Acid fuchsin
Regarding the counterstain for use with Sanderson's Rapid Bone Stain, I use the Van Gieson picrofuchsin in that it will stain older, mature, mineralized bone red. As an additional consequence of the VG counterstain, the originally blue stained dense collagen (new bone/ osteoid) from the SRBS will change to a greenish color from the reaction of the yellow picric acid (present in the counterstain). If you think about it, it makes perfect sense due to the basic reaction of combining blue and yellow to yield green. Additionally, the intensity of the red from the acid fuchsin content of the counterstain is directly proportional to the mineral density of the bone due to the acid in the solution acting to lightly etch the mineralized bone. Also, think of the specimen in 3D, you could expect shades of red depending on the plane of sectioning within the specimen and depending upon mineral deposition at the surface of trabeculae. Therefore, I believe that the dye-tissue interaction you are inquiring about is associated to mineralization or calcium concentration. Hope this helps to answer your question. Jack On Dec 21, 2009, at 1:25 PM, sk...@illinois.edu wrote: Hi All - I am trying to figure out if the combination of Sanderson's rapid bone stain and acid fuchsin as the counterstain (or methylene blue + basic fuchsin) results in pink/red staining of biologically formed apatite (apatite not associated with collagen produced by osteoblasts)? An associated question is if the dye-tissue interaction between acid or basic fuchsin and mineralized bone is due to dye-collagen interactions? If anyone has any experiences or references that may help, please let me know. Thank you for your help, Sheeny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
