Re: [Histonet] So... I Need Some Expert Advice:
I suggest to start a board/forum. Really. On Wed, 1 Apr 2009 23:13:09 -0700 (PDT), aa aa lactose.intoler...@yahoo.com wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice:
What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] So... I Need Some Expert Advice:
Mark, Histology, who cares, turned up the volume and danced round the office singing along to this Stock, Aitken and Waterman classic. This soul boy even has the album on his iPhone. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Ray Sent: 02 April 2009 10:10 To: lactose.intoler...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] So... I Need Some Expert Advice: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] So... I Need Some Expert Advice:
I think it was April fool... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Ray Sent: Thursday, April 02, 2009 5:10 AM To: lactose.intoler...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] So... I Need Some Expert Advice: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice:
Looked like a scam to me so I hit Delete immediately. No real name, no real address = scam Geoff Mark Ray wrote: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice:
Looked like a scam to me so I hit Delete immediately. No real name, no real address = scam It was a link to a video, I believe, not a scam. From: Geoff McAuliffe mcaul...@umdnj.edu To: Mark Ray darkd...@comcast.net Cc: histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 10:12:30 AM Subject: Re: [Histonet] So... I Need Some Expert Advice: Looked like a scam to me so I hit Delete immediately. No real name, no real address = scam Geoff Mark Ray wrote: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice:
It wasn't funky it was a good video - but it had no relevance to what we are supposed to be doing here on histonet. Now if somebody could post the link to some Adam Lambert tunes... Andi;-) On Apr 2, 2009, at 2:10 AM, Mark Ray wrote: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice:
Come on people. Yesterday was April Fool's Day and Histonet got Rick Rolled. Today is the 2nd, move on. PKP From: Andrea Grantham algra...@email.arizona.edu To: HISTONET histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 9:33:06 AM Subject: Re: [Histonet] So... I Need Some Expert Advice: It wasn't funky it was a good video - but it had no relevance to what we are supposed to be doing here on histonet. Now if somebody could post the link to some Adam Lambert tunes... Andi;-) On Apr 2, 2009, at 2:10 AM, Mark Ray wrote: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] So... I Need Some Expert Advice:
Might I suggest that what we need on Histonet is to deal with some basic questions in histology that have never been answered in detail but have only been touched upon. Histonet has a great deal of expertise and it seems we should be asking these major questions. Topics such as what is optimal time of fixing, best waxes etc. and why should be issues here. My perception is that we often will give personal opinions as to what the best techniques or materials are but not the science behind the rational for choosing these, maybe because we do not know. However I feel that this is an area that we need to expand. Hope that this makes sense. I've had a week of family medical issues, broken plumbing under the house (guess that better than personal plumbing being broken!), two computer crashes and fire ants to name only the major ones. There is therefore, in my opinion, no way to go but up from here so can we hear your views please? Barry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] So... I Need Some Expert Advice:
I'm not in total disagreement with Barry. The FAQ is a good place to start, and it might be all we need, but I wonder it shouldn't be revisited just to be sure. There seems to be a lot of questions lately on some of the basics as well as queries about instrumentation. My question would be this: In light of different internet technologies, would a blog format be an acceptable way of handling or grouping some of these issues? I'm certainly not advocating a change in Histonet, as it is a fine format that is serving the community well. I'm just offering this as a possibile answer to Barry's concern, if others find his concern worth looking into. Just a thought. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Thursday, April 02, 2009 10:45 AM To: HISTONET Subject: RE: [Histonet] So... I Need Some Expert Advice: Might I suggest that what we need on Histonet is to deal with some basic questions in histology that have never been answered in detail but have only been touched upon. Histonet has a great deal of expertise and it seems we should be asking these major questions. Topics such as what is optimal time of fixing, best waxes etc. and why should be issues here. My perception is that we often will give personal opinions as to what the best techniques or materials are but not the science behind the rational for choosing these, maybe because we do not know. However I feel that this is an area that we need to expand. Hope that this makes sense. I've had a week of family medical issues, broken plumbing under the house (guess that better than personal plumbing being broken!), two computer crashes and fire ants to name only the major ones. There is therefore, in my opinion, no way to go but up from here so can we hear your views please? Barry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice (forum for information):
True true that would be cool. But I don't mind repeated topics being discussed again and again - it isn't always a bad thing, as although histology is a very old and largely well-established science, there is room for improvements and modifications over time.so I appreciate the ongoing discussions over the years, even if a lot of things get repeated. And there ARE a lot of protocols and stable forum websites out there for histo stuff. As fab as Histonet is, it is one of many online resources I consult daily. Andrea -Original Message- From: Nicole Collette collet...@mail.llnl.gov Date: Thu, 02 Apr 2009 09:43:04 To: HISTONEThistonet@lists.utsouthwestern.edu Subject: RE: [Histonet] So... I Need Some Expert Advice (forum for information): Hi, All, I'm not generally one to put in my two cents' worth, but today I'm feeling especially chipper. In my world, (idealistic optimist that I am), I would love to see something like a cross between PLoS One, Wikipedia, and Cold Spring Harbor protocols- A mainstream, accepted (or widely used but still not definitive) protocol, or commonly used equipment, with links to discussion for individual steps or reagents. For example, fixing tissue would list a set of reagents, a basic process, and reagents or steps that are often up for debate (Why use NBF? what is paraformaldehyde? what does glutaraldehyde do? How do you get that picric acid yellow out?) would be associated with a link to discussion, so that people who are just looking for the facts can have them readily available, without side comments, and those who want to know the nitty gritty details that cause controversy can make their own judgements, or add their own comments. Now, I'm not at all technologically able to build this resource, but would give anyone who wanted to take it on an Attaboy! or Attagirl! And if you do, please send me the link ;) There's a lot of good info here, and some really great expertise, but finding it threaded through the archives is not the easiest way to find this stuff, and it is evident by the discussions that come up over and over. Thanks for listening! Keep up the good work! Sincerely, Nicole Collette _ At 10:13 AM -0600 4/2/09, O'Donnell, Bill wrote: I'm not in total disagreement with Barry. The FAQ is a good place to start, and it might be all we need, but I wonder it shouldn't be revisited just to be sure. There seems to be a lot of questions lately on some of the basics as well as queries about instrumentation. My question would be this: In light of different internet technologies, would a blog format be an acceptable way of handling or grouping some of these issues? I'm certainly not advocating a change in Histonet, as it is a fine format that is serving the community well. I'm just offering this as a possibile answer to Barry's concern, if others find his concern worth looking into. Just a thought. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Thursday, April 02, 2009 10:45 AM To: HISTONET Subject: RE: [Histonet] So... I Need Some Expert Advice: Might I suggest that what we need on Histonet is to deal with some basic questions in histology that have never been answered in detail but have only been touched upon. Histonet has a great deal of expertise and it seems we should be asking these major questions. Topics such as what is optimal time of fixing, best waxes etc. and why should be issues here. My perception is that we often will give personal opinions as to what the best techniques or materials are but not the science behind the rational for choosing these, maybe because we do not know. However I feel that this is an area that we need to expand. Hope that this makes sense. I've had a week of family medical issues, broken plumbing under the house (guess that better than personal plumbing being broken!), two computer crashes and fire ants to name only the major ones. There is therefore, in my opinion, no way to go but up from here so can we hear your views please? Barry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http:// lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http:// lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] So... I Need Some Expert Advice:
Barry: Agree. Pick up ONE theme and ask for everybody to contribute. If satisfactorily covered, pick another and so on. René J. --- On Thu, 4/2/09, Rittman, Barry R barry.r.ritt...@uth.tmc.edu wrote: From: Rittman, Barry R barry.r.ritt...@uth.tmc.edu Subject: RE: [Histonet] So... I Need Some Expert Advice: To: HISTONET histonet@lists.utsouthwestern.edu Date: Thursday, April 2, 2009, 11:44 AM Might I suggest that what we need on Histonet is to deal with some basic questions in histology that have never been answered in detail but have only been touched upon. Histonet has a great deal of expertise and it seems we should be asking these major questions. Topics such as what is optimal time of fixing, best waxes etc. and why should be issues here. My perception is that we often will give personal opinions as to what the best techniques or materials are but not the science behind the rational for choosing these, maybe because we do not know. However I feel that this is an area that we need to expand. Hope that this makes sense. I've had a week of family medical issues, broken plumbing under the house (guess that better than personal plumbing being broken!), two computer crashes and fire ants to name only the major ones. There is therefore, in my opinion, no way to go but up from here so can we hear your views please? Barry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
