[Histonet] Start up lab
I started a lab with the help of Miraca Life Science. Policies and procedures is probably the toughest. Finding a vender to take xylene waste an getting haz waste credentials. CLIA certifies & monitors the lab. I used SharpsInc.com for my biohazard as my volume was to low to use stericycle and not cost effective. doing everything yourself is not easy but can be done but your output is low due to the amount of taskes to be completed. hope that helps. Brent @ Acadiana Gastroenterology Assc Lafayette, LA Sent from my Verizon, Samsung Galaxy smartphone Original message From: histonet-requ...@lists.utsouthwestern.edu Date: 7/10/16 12:16 PM (GMT-06:00) To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 152, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. microtome validation (Ann Specian) 2. Re: microtome validation (Jacox, Robert A.) 3. Start up Vet/Animal histology laboratory (Linda) -- Message: 1 Date: Sat, 9 Jul 2016 13:15:43 -0400 From: Ann SpecianTo: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome validation Message-ID: <155d0a85e33-212e-1d...@webprd-m79.mail.aol.com> Content-Type: text/plain; charset=utf-8 Has anyone had to validate a microtome (manual/automatic). If so, how did you do it? Ann -- Message: 2 Date: Sat, 9 Jul 2016 17:53:24 + From: "Jacox, Robert A." To: Ann Specian Cc: "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] microtome validation Message-ID: Content-Type: text/plain; charset="us-ascii" Ann, I would recommend validating manual microtomes more often then automatic. Any traditional gear driven microtome does change overtime as gears wear. This eventually will lead to the micron indicator on the microtome not reading what you are truly cutting. Automated, microtomes driven by stepper motors tend to remain accurate longer but you may want to document the accuracy every couple of years. To check calibration most service engineers will mount a micrometer to the microtome and measure the feed of the microtome as the hand wheel turns. The procedure generally takes 5 minutes. Check with your microtome manufacture to see if they can assist you. Robert Jacox Thermo Fisher Scientific Commercial Marketing Manager Sent from my iPad > On Jul 9, 2016, at 1:39 PM, Ann Specian via Histonet > wrote: > > Has anyone had to validate a microtome (manual/automatic). If so, how did > you do it? > Ann > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Sat, 9 Jul 2016 23:53:23 + (UTC) From: Linda To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Start up Vet/Animal histology laboratory Message-ID: <1990575214.1453277.1468108403616.javamail.ya...@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Hello Histoland, I am inquiring if anyone has started their own lab to?do histology services for veterinarians?or animal research?tissues? Or anyone who has started a private lab? What are your experiences?? What didn't you expect?? Thank you in advance. Regards, Linda Dee, BGS,HT(ASCP)?lmd...@yahoo.com -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- End of Histonet Digest, Vol 152, Issue 8 PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. ___
RE: [Histonet] Start Up Lab
well...maybe it's just research labs at UB then...i guess we're pretty old school! --On Wednesday, July 22, 2009 7:03 PM -0400 Blazek, Linda lbla...@digestivespecialists.com wrote: Hay! Another one of us oldies! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Wednesday, July 22, 2009 6:07 PM To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
You still have fingers!?. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
Ah, yes. The '70's. Our lab had 2 windows, both on the same side of the wall. The pathologists cut under one of the windows and it had one of those box fans, pointing out. In Michigan. No hoods, an old duo-autotechnicon and xylene in full use with disposal down the drain. Every day, I would go home not knowing if I went thru a red light or not, arriving home , head still cloudy for about 30 minutes until it cleared. Ah..those were the days?? Although I miss it, I'm glad I had to give up my coffee and donut when cutting. Treats in the drawer are gone, mouth pipetting is gone (not mine, but yes, the mouth does turn black from silver nitrate!!) Progress is good. But I still like my '70's music!!! Bob Seger and old motown!!! ;) Montina Van Meter montina.vanme...@pbrc.edu 07/23/09 11:31 AM I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent. I think you mean dioxane. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah! Sure do. -Original Message- From: Edwards, R.E. [mailto:r...@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http
RE: [Histonet] Start Up Lab
Thanks to spell-check..it self-corrected me..incorrectly! Thanks for setting it right. -Original Message- From: Smith, Allen [mailto:asm...@mail.barry.edu] Sent: Thursday, July 23, 2009 12:26 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Start Up Lab I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent. I think you mean dioxane. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah! Sure do. -Original Message- From: Edwards, R.E. [mailto:r...@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However
RE: [Histonet] Start Up Lab
In the 80's I worked in a drug screening lab for the Navy and one step in the extraction involved fuming HCl (meaning pure HCl). Several of the techs got nose bleeds every time they used the stuff. I finally pried open a window (they were painted shut) and we turned on a fan the size of a propeller to blow the fumes out the window. We used Xylene in a room with no ventilation and found out that high concentrations of xylene cause transient euphoria. No wonder we thought every thing was funny during those days. Safety people, they take all the fun out of research! ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 Your images flow through our CRIC. --- On Thu, 7/23/09, Lynette Pavelich lpave...@hurleymc.com wrote: From: Lynette Pavelich lpave...@hurleymc.com Subject: RE: [Histonet] Start Up Lab To: cb...@lexclin.com, montina.vanme...@pbrc.edu, theci...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Date: Thursday, July 23, 2009, 3:53 PM Ah, yes. The '70's. Our lab had 2 windows, both on the same side of the wall. The pathologists cut under one of the windows and it had one of those box fans, pointing out. In Michigan. No hoods, an old duo-autotechnicon and xylene in full use with disposal down the drain. Every day, I would go home not knowing if I went thru a red light or not, arriving home , head still cloudy for about 30 minutes until it cleared. Ah..those were the days?? Although I miss it, I'm glad I had to give up my coffee and donut when cutting. Treats in the drawer are gone, mouth pipetting is gone (not mine, but yes, the mouth does turn black from silver nitrate!!) Progress is good. But I still like my '70's music!!! Bob Seger and old motown!!! ;) Montina Van Meter montina.vanme...@pbrc.edu 07/23/09 11:31 AM I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing
RE: [Histonet] Start Up Lab
You might still have your fingers, but are your fingerprints still the same? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 Your images flow through our CRIC. --- On Thu, 7/23/09, Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov wrote: From: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov Subject: RE: [Histonet] Start Up Lab To: Smith, Allen asm...@mail.barry.edu Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, July 23, 2009, 4:32 PM Thanks to spell-check..it self-corrected me..incorrectly! Thanks for setting it right. -Original Message- From: Smith, Allen [mailto:asm...@mail.barry.edu] Sent: Thursday, July 23, 2009 12:26 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Start Up Lab I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent. I think you mean dioxane. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah! Sure do. -Original Message- From: Edwards, R.E. [mailto:r...@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot
RE: [Histonet] Start Up Lab
I went to the Navy's MLT program in 79-80 and we did drug screens manually and the chemicals that we used would give us a buzz. Thought it was ironic that I was getting high while looking for drugs in somebody else. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello [vapat...@yahoo.com] Sent: Thursday, July 23, 2009 1:30 PM To: Lynette Pavelich; HistoNet Subject: RE: [Histonet] Start Up Lab In the 80's I worked in a drug screening lab for the Navy and one step in the extraction involved fuming HCl (meaning pure HCl). Several of the techs got nose bleeds every time they used the stuff. I finally pried open a window (they were painted shut) and we turned on a fan the size of a propeller to blow the fumes out the window. We used Xylene in a room with no ventilation and found out that high concentrations of xylene cause transient euphoria. No wonder we thought every thing was funny during those days. Safety people, they take all the fun out of research! ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 Your images flow through our CRIC. --- On Thu, 7/23/09, Lynette Pavelich lpave...@hurleymc.com wrote: From: Lynette Pavelich lpave...@hurleymc.com Subject: RE: [Histonet] Start Up Lab To: cb...@lexclin.com, montina.vanme...@pbrc.edu, theci...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Date: Thursday, July 23, 2009, 3:53 PM Ah, yes. The '70's. Our lab had 2 windows, both on the same side of the wall. The pathologists cut under one of the windows and it had one of those box fans, pointing out. In Michigan. No hoods, an old duo-autotechnicon and xylene in full use with disposal down the drain. Every day, I would go home not knowing if I went thru a red light or not, arriving home , head still cloudy for about 30 minutes until it cleared. Ah..those were the days?? Although I miss it, I'm glad I had to give up my coffee and donut when cutting. Treats in the drawer are gone, mouth pipetting is gone (not mine, but yes, the mouth does turn black from silver nitrate!!) Progress is good. But I still like my '70's music!!! Bob Seger and old motown!!! ;) Montina Van Meter montina.vanme...@pbrc.edu 07/23/09 11:31 AM I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http
[Histonet] Start Up Lab
And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Start Up Lab
I would add an eye wash station and a chemical spill kit to the list. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
(lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Start Up Lab
Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet