[Histonet] Start up lab

2016-07-10 Thread Brent Adams via Histonet 

I started a lab with the help of Miraca Life Science. Policies and procedures 
is probably the toughest. Finding a vender to take xylene waste an getting haz 
waste credentials. CLIA certifies  & monitors the lab. I used SharpsInc.com for 
my biohazard as my volume was to low to use stericycle and not cost effective. 
doing everything yourself is not easy but can be done but your output is low 
due to the amount of taskes to be completed.

hope that helps.
Brent @ Acadiana Gastroenterology Assc
Lafayette, LA
Sent from my Verizon, Samsung Galaxy smartphone


 Original message 
From: histonet-requ...@lists.utsouthwestern.edu
Date: 7/10/16 12:16 PM (GMT-06:00)
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 152, Issue 8

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Today's Topics:

   1. microtome validation (Ann Specian)
   2. Re: microtome validation (Jacox, Robert A.)
   3. Start up Vet/Animal histology laboratory (Linda)


--

Message: 1
Date: Sat, 9 Jul 2016 13:15:43 -0400
From: Ann Specian 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome validation
Message-ID: <155d0a85e33-212e-1d...@webprd-m79.mail.aol.com>
Content-Type: text/plain; charset=utf-8

Has anyone had to validate a microtome (manual/automatic).  If so, how did you 
do it?
Ann


--

Message: 2
Date: Sat, 9 Jul 2016 17:53:24 +
From: "Jacox, Robert A." 
To: Ann Specian 
Cc: "histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] microtome validation
Message-ID: 
Content-Type: text/plain; charset="us-ascii"

Ann,

I would recommend validating manual microtomes more often then automatic. Any 
traditional gear driven microtome does change overtime as gears wear. This 
eventually will lead to the micron indicator on the microtome not reading what 
you are truly cutting. Automated, microtomes driven by stepper motors tend to 
remain accurate longer but you may want to document the accuracy every couple 
of years.

To check calibration most service engineers will mount a micrometer to the 
microtome and measure the feed of the microtome as the hand wheel turns. The 
procedure generally takes 5 minutes. Check with your microtome manufacture to 
see if they can assist you.

Robert Jacox
Thermo Fisher Scientific
Commercial Marketing Manager



Sent from my iPad

> On Jul 9, 2016, at 1:39 PM, Ann Specian via Histonet 
>  wrote:
>
> Has anyone had to validate a microtome (manual/automatic).  If so, how did 
> you do it?
> Ann
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--

Message: 3
Date: Sat, 9 Jul 2016 23:53:23 + (UTC)
From: Linda 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Start up Vet/Animal histology laboratory
Message-ID:
<1990575214.1453277.1468108403616.javamail.ya...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

 Hello Histoland,
I am inquiring if anyone has started their own lab to?do histology services for 
veterinarians?or animal research?tissues?
Or anyone who has started a private lab?
What are your experiences?? What didn't you expect??
Thank you in advance.
Regards,
Linda Dee, BGS,HT(ASCP)?lmd...@yahoo.com



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RE: [Histonet] Start Up Lab

2009-07-23 Thread Merced M Leiker 
well...maybe it's just research labs at UB then...i guess we're pretty 
old school!


--On Wednesday, July 22, 2009 7:03 PM -0400 Blazek, Linda 
lbla...@digestivespecialists.com wrote:



Hay!  Another one of us oldies!

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lbla...@digestivespecialists.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette
Pavelich Sent: Wednesday, July 22, 2009 6:07 PM
To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu;
cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab

Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..


Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 

(lol some labs have a bench area as well as a desk area where food is
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire
cing...@uwhealth.org wrote:


In the lab?!? For shame. :)



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy

DuBois

Sent: Wed 7/22/2009 10:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Start Up Lab



And a coffee pot.

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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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RE: [Histonet] Start Up Lab

2009-07-23 Thread Carol Bryant 
Love it!  Too funny! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
theci...@yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed 
tissue adds a certain je ne sais quoi to the flavor. :/
Sent from my Verizon Wireless BlackBerry

-Original Message-
From: Lynette Pavelich lpave...@hurleymc.com

Date: Wed, 22 Jul 2009 18:07:07 
To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; 
cing...@uwhealth.org
Subject: RE: [Histonet] Start Up Lab


Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..

 Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 
(lol some labs have a bench area as well as a desk area where food is 
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
cing...@uwhealth.org wrote:

 In the lab?!? For shame. :)

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy
DuBois
 Sent: Wed 7/22/2009 10:29 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Start Up Lab



 And a coffee pot.

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet



 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


___
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RE: [Histonet] Start Up Lab

2009-07-23 Thread Montina Van Meter 
I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their personal drawer.  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.  


 

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
theci...@yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/
Sent from my Verizon Wireless BlackBerry

-Original Message-
From: Lynette Pavelich lpave...@hurleymc.com

Date: Wed, 22 Jul 2009 18:07:07 
To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu;
cing...@uwhealth.org
Subject: RE: [Histonet] Start Up Lab


Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..

 Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 
(lol some labs have a bench area as well as a desk area where food is 
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
cing...@uwhealth.org wrote:

 In the lab?!? For shame. :)

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy
DuBois
 Sent: Wed 7/22/2009 10:29 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Start Up Lab



 And a coffee pot.

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet



 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


___
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RE: [Histonet] Start Up Lab

2009-07-23 Thread Bartlett, Jeanine (CDC/CCID/NCZVED) 
And I used to use dioxin in my tissue processor and would actually dip
gauze in it to clean paraffin off my fingers. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina
Van Meter
Sent: Thursday, July 23, 2009 11:31 AM
To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their personal drawer.  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.  


 

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
theci...@yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent
from my Verizon Wireless BlackBerry

-Original Message-
From: Lynette Pavelich lpave...@hurleymc.com

Date: Wed, 22 Jul 2009 18:07:07
To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu;
cing...@uwhealth.org
Subject: RE: [Histonet] Start Up Lab


Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..

 Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 
(lol some labs have a bench area as well as a desk area where food is 
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
cing...@uwhealth.org wrote:

 In the lab?!? For shame. :)

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy
DuBois
 Sent: Wed 7/22/2009 10:29 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Start Up Lab



 And a coffee pot.

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet



 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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RE: [Histonet] Start Up Lab

2009-07-23 Thread Edwards, R.E. 
You still  have  fingers!?.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, 
Jeanine (CDC/CCID/NCZVED)
Sent: 23 July 2009 16:44
To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

And I used to use dioxin in my tissue processor and would actually dip
gauze in it to clean paraffin off my fingers. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina
Van Meter
Sent: Thursday, July 23, 2009 11:31 AM
To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their personal drawer.  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.  


 

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
theci...@yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent
from my Verizon Wireless BlackBerry

-Original Message-
From: Lynette Pavelich lpave...@hurleymc.com

Date: Wed, 22 Jul 2009 18:07:07
To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu;
cing...@uwhealth.org
Subject: RE: [Histonet] Start Up Lab


Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..

 Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 
(lol some labs have a bench area as well as a desk area where food is 
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
cing...@uwhealth.org wrote:

 In the lab?!? For shame. :)

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy
DuBois
 Sent: Wed 7/22/2009 10:29 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Start Up Lab



 And a coffee pot.

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet



 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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RE: [Histonet] Start Up Lab

2009-07-23 Thread Lynette Pavelich 
Ah, yes.  The '70's.  Our lab had 2 windows, both on the same side of
the wall.  The pathologists cut under one of the windows and it had one
of those box fans, pointing out.  In Michigan.  No hoods, an old
duo-autotechnicon and xylene in full use with disposal down the drain. 
Every day, I would go home not knowing if I went thru a red light or
not, arriving home , head still cloudy for about 30 minutes until it
cleared.  Ah..those were the days??  Although I miss it, I'm glad I had
to give up my coffee and donut when cutting.  Treats in the drawer are
gone, mouth pipetting is gone (not mine, but yes, the mouth does turn
black from silver nitrate!!)  Progress is good.  But I still like my
'70's music!!!  Bob Seger and old motown!!! ;)

 Montina Van Meter montina.vanme...@pbrc.edu 07/23/09 11:31 AM

I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their personal drawer.  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.  


 

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
theci...@yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/
Sent from my Verizon Wireless BlackBerry

-Original Message-
From: Lynette Pavelich lpave...@hurleymc.com

Date: Wed, 22 Jul 2009 18:07:07 
To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu;
cing...@uwhealth.org
Subject: RE: [Histonet] Start Up Lab


Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..

 Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 
(lol some labs have a bench area as well as a desk area where food is 
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
cing...@uwhealth.org wrote:

 In the lab?!? For shame. :)

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy
DuBois
 Sent: Wed 7/22/2009 10:29 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Start Up Lab



 And a coffee pot.

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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RE: [Histonet] Start Up Lab

2009-07-23 Thread Smith, Allen 
I can't imagine why any one would use dioxin, which used to be used as an 
insulator and heat convector in electron microscope transformers, as a clearing 
agent.  I think you mean dioxane.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, 
Jeanine (CDC/CCID/NCZVED)
Sent: Thursday, July 23, 2009 12:13 PM
To: Edwards, R.E.; Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette 
Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Hah!  Sure do. 




-Original Message-
From: Edwards, R.E. [mailto:r...@leicester.ac.uk] 
Sent: Thursday, July 23, 2009 11:51 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol
Bryant; theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

You still  have  fingers!?.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: 23 July 2009 16:44
To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette
Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

And I used to use dioxin in my tissue processor and would actually dip
gauze in it to clean paraffin off my fingers. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina
Van Meter
Sent: Thursday, July 23, 2009 11:31 AM
To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their personal drawer.  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.  


 

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
theci...@yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent
from my Verizon Wireless BlackBerry

-Original Message-
From: Lynette Pavelich lpave...@hurleymc.com

Date: Wed, 22 Jul 2009 18:07:07
To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu;
cing...@uwhealth.org
Subject: RE: [Histonet] Start Up Lab


Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..

 Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 
(lol some labs have a bench area as well as a desk area where food is 
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
cing...@uwhealth.org wrote:

 In the lab?!? For shame. :)

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy
DuBois
 Sent: Wed 7/22/2009 10:29 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Start Up Lab



 And a coffee pot.

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet



 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http

RE: [Histonet] Start Up Lab

2009-07-23 Thread Bartlett, Jeanine (CDC/CCID/NCZVED) 
Thanks to spell-check..it self-corrected me..incorrectly!
Thanks for setting it right.



-Original Message-
From: Smith, Allen [mailto:asm...@mail.barry.edu] 
Sent: Thursday, July 23, 2009 12:26 PM
To: Bartlett, Jeanine (CDC/CCID/NCZVED)
Cc: 'Histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Start Up Lab

I can't imagine why any one would use dioxin, which used to be used as
an insulator and heat convector in electron microscope transformers, as
a clearing agent.  I think you mean dioxane.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: Thursday, July 23, 2009 12:13 PM
To: Edwards, R.E.; Montina Van Meter; Carol Bryant; theci...@yahoo.com;
Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Hah!  Sure do. 




-Original Message-
From: Edwards, R.E. [mailto:r...@leicester.ac.uk]
Sent: Thursday, July 23, 2009 11:51 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol
Bryant; theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

You still  have  fingers!?.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: 23 July 2009 16:44
To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette
Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

And I used to use dioxin in my tissue processor and would actually dip
gauze in it to clean paraffin off my fingers. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina
Van Meter
Sent: Thursday, July 23, 2009 11:31 AM
To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their personal drawer.  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.  


 

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
theci...@yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent
from my Verizon Wireless BlackBerry

-Original Message-
From: Lynette Pavelich lpave...@hurleymc.com

Date: Wed, 22 Jul 2009 18:07:07
To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu;
cing...@uwhealth.org
Subject: RE: [Histonet] Start Up Lab


Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..

 Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 
(lol some labs have a bench area as well as a desk area where food is
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire
cing...@uwhealth.org wrote:

 In the lab?!? For shame. :)

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy
DuBois
 Sent: Wed 7/22/2009 10:29 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Start Up Lab



 And a coffee pot.

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet



 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However

RE: [Histonet] Start Up Lab

2009-07-23 Thread Va Paula Sicurello 
In the 80's I worked in a drug screening lab for the Navy and one step in the 
extraction involved fuming HCl (meaning pure HCl).  Several of the techs got 
nose bleeds every time they used the stuff.  I finally pried open a window 
(they were painted shut) and we turned on a fan the size of a propeller to blow 
the fumes out the window.

We used Xylene in a room with no ventilation and found out that high 
concentrations of xylene cause transient euphoria.  No wonder we thought 
every thing was funny during those days.

Safety people, they take all the fun out of research!  ;-)

Paula Sicurello

VA Medical Center San Diego

Veterans Medical Research Foundation (VMRF) 

Core Research Imaging Center (CRIC)

3350 La Jolla Village Dr., MC151

San Diego, CA 92161

858-552-8585 x2397



Your images flow through our CRIC.

--- On Thu, 7/23/09, Lynette Pavelich lpave...@hurleymc.com wrote:

From: Lynette Pavelich lpave...@hurleymc.com
Subject: RE: [Histonet] Start Up Lab
To: cb...@lexclin.com, montina.vanme...@pbrc.edu, theci...@yahoo.com
Cc: histonet@lists.utsouthwestern.edu
Date: Thursday, July 23, 2009, 3:53 PM

Ah, yes.  The '70's.  Our lab had 2 windows, both on the same side of
the wall.  The pathologists cut under one of the windows and it had one
of those box fans, pointing out.  In Michigan.  No hoods, an old
duo-autotechnicon and xylene in full use with disposal down the drain. 
Every day, I would go home not knowing if I went thru a red light or
not, arriving home , head still cloudy for about 30 minutes until it
cleared.  Ah..those were the days??  Although I miss it, I'm glad I had
to give up my coffee and donut when cutting.  Treats in the drawer are
gone, mouth pipetting is gone (not mine, but yes, the mouth does turn
black from silver nitrate!!)  Progress is good.  But I still like my
'70's music!!!  Bob Seger and old motown!!! ;)

 Montina Van Meter montina.vanme...@pbrc.edu 07/23/09 11:31 AM

I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their personal drawer.  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.  


 

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
theci...@yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/
Sent from my Verizon Wireless BlackBerry

-Original Message-
From: Lynette Pavelich lpave...@hurleymc.com

Date: Wed, 22 Jul 2009 18:07:07 
To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu;
cing...@uwhealth.org
Subject: RE: [Histonet] Start Up Lab


Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..

 Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 
(lol some labs have a bench area as well as a desk area where food is 
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
cing...@uwhealth.org wrote:

 In the lab?!? For shame. :)

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy
DuBois
 Sent: Wed 7/22/2009 10:29 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Start Up Lab



 And a coffee pot.

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet



 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


___
Histonet mailing

RE: [Histonet] Start Up Lab

2009-07-23 Thread Va Paula Sicurello 
You might still have your fingers, but are your fingerprints still the same?  

Paula Sicurello

VA Medical Center San Diego

Veterans Medical Research Foundation (VMRF) 

Core Research Imaging Center (CRIC)

3350 La Jolla Village Dr., MC151

San Diego, CA 92161

858-552-8585 x2397



Your images flow through our CRIC.

--- On Thu, 7/23/09, Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov wrote:

From: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov
Subject: RE: [Histonet] Start Up Lab
To: Smith, Allen asm...@mail.barry.edu
Cc: Histonet@lists.utsouthwestern.edu
Date: Thursday, July 23, 2009, 4:32 PM

Thanks to spell-check..it self-corrected me..incorrectly!
Thanks for setting it right.



-Original Message-
From: Smith, Allen [mailto:asm...@mail.barry.edu] 
Sent: Thursday, July 23, 2009 12:26 PM
To: Bartlett, Jeanine (CDC/CCID/NCZVED)
Cc: 'Histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Start Up Lab

I can't imagine why any one would use dioxin, which used to be used as
an insulator and heat convector in electron microscope transformers, as
a clearing agent.  I think you mean dioxane.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: Thursday, July 23, 2009 12:13 PM
To: Edwards, R.E.; Montina Van Meter; Carol Bryant; theci...@yahoo.com;
Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Hah!  Sure do. 




-Original Message-
From: Edwards, R.E. [mailto:r...@leicester.ac.uk]
Sent: Thursday, July 23, 2009 11:51 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol
Bryant; theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

You still  have  fingers!?.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: 23 July 2009 16:44
To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette
Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

And I used to use dioxin in my tissue processor and would actually dip
gauze in it to clean paraffin off my fingers. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina
Van Meter
Sent: Thursday, July 23, 2009 11:31 AM
To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their personal drawer.  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.  


 

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
theci...@yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent
from my Verizon Wireless BlackBerry

-Original Message-
From: Lynette Pavelich lpave...@hurleymc.com

Date: Wed, 22 Jul 2009 18:07:07
To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu;
cing...@uwhealth.org
Subject: RE: [Histonet] Start Up Lab


Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..

 Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 
(lol some labs have a bench area as well as a desk area where food is
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire
cing...@uwhealth.org wrote:

 In the lab?!? For shame. :)

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy
DuBois
 Sent: Wed 7/22/2009 10:29 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Start Up Lab



 And a coffee pot

RE: [Histonet] Start Up Lab

2009-07-23 Thread Podawiltz, Thomas 
I went to the Navy's MLT program in 79-80 and we did drug screens manually and 
the chemicals that we used would give us a buzz. Thought it was ironic that I 
was getting high while looking for drugs in somebody else.


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello 
[vapat...@yahoo.com]
Sent: Thursday, July 23, 2009 1:30 PM
To: Lynette Pavelich; HistoNet
Subject: RE: [Histonet] Start Up Lab

In the 80's I worked in a drug screening lab for the Navy and one step in the 
extraction involved fuming HCl (meaning pure HCl).  Several of the techs got 
nose bleeds every time they used the stuff.  I finally pried open a window 
(they were painted shut) and we turned on a fan the size of a propeller to blow 
the fumes out the window.

We used Xylene in a room with no ventilation and found out that high 
concentrations of xylene cause transient euphoria.  No wonder we thought 
every thing was funny during those days.

Safety people, they take all the fun out of research!  ;-)

Paula Sicurello

VA Medical Center San Diego

Veterans Medical Research Foundation (VMRF)

Core Research Imaging Center (CRIC)

3350 La Jolla Village Dr., MC151

San Diego, CA 92161

858-552-8585 x2397



Your images flow through our CRIC.

--- On Thu, 7/23/09, Lynette Pavelich lpave...@hurleymc.com wrote:

From: Lynette Pavelich lpave...@hurleymc.com
Subject: RE: [Histonet] Start Up Lab
To: cb...@lexclin.com, montina.vanme...@pbrc.edu, theci...@yahoo.com
Cc: histonet@lists.utsouthwestern.edu
Date: Thursday, July 23, 2009, 3:53 PM

Ah, yes.  The '70's.  Our lab had 2 windows, both on the same side of
the wall.  The pathologists cut under one of the windows and it had one
of those box fans, pointing out.  In Michigan.  No hoods, an old
duo-autotechnicon and xylene in full use with disposal down the drain.
Every day, I would go home not knowing if I went thru a red light or
not, arriving home , head still cloudy for about 30 minutes until it
cleared.  Ah..those were the days??  Although I miss it, I'm glad I had
to give up my coffee and donut when cutting.  Treats in the drawer are
gone, mouth pipetting is gone (not mine, but yes, the mouth does turn
black from silver nitrate!!)  Progress is good.  But I still like my
'70's music!!!  Bob Seger and old motown!!! ;)

 Montina Van Meter montina.vanme...@pbrc.edu 07/23/09 11:31 AM

I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their personal drawer.  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.




Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
theci...@yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/
Sent from my Verizon Wireless BlackBerry

-Original Message-
From: Lynette Pavelich lpave...@hurleymc.com

Date: Wed, 22 Jul 2009 18:07:07
To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu;
cing...@uwhealth.org
Subject: RE: [Histonet] Start Up Lab


Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..

 Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 
(lol some labs have a bench area as well as a desk area where food is
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire
cing...@uwhealth.org wrote:

 In the lab?!? For shame. :)

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy
DuBois
 Sent: Wed 7/22/2009 10:29 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Start Up Lab



 And a coffee pot.

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[Histonet] Start Up Lab

2009-07-22 Thread Cindy DuBois 
And a coffee pot.

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[Histonet] Start Up Lab

2009-07-22 Thread Perry, Margaret 
I would add an eye wash station and a chemical spill kit to the list.

Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007

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RE: [Histonet] Start Up Lab

2009-07-22 Thread Ingles Claire 
In the lab?!? For shame. :)



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois
Sent: Wed 7/22/2009 10:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Start Up Lab



And a coffee pot.

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RE: [Histonet] Start Up Lab

2009-07-22 Thread Merced M Leiker 
(lol some labs have a bench area as well as a desk area where food is 
allowed.)


--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
cing...@uwhealth.org wrote:



In the lab?!? For shame. :)



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois
Sent: Wed 7/22/2009 10:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Start Up Lab



And a coffee pot.

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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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RE: [Histonet] Start Up Lab

2009-07-22 Thread Lynette Pavelich 
Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..

 Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 
(lol some labs have a bench area as well as a desk area where food is 
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
cing...@uwhealth.org wrote:

 In the lab?!? For shame. :)

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy
DuBois
 Sent: Wed 7/22/2009 10:29 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Start Up Lab



 And a coffee pot.

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet



 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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Re: [Histonet] Start Up Lab

2009-07-22 Thread thecitan 
Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed 
tissue adds a certain je ne sais quoi to the flavor. :/
Sent from my Verizon Wireless BlackBerry

-Original Message-
From: Lynette Pavelich lpave...@hurleymc.com

Date: Wed, 22 Jul 2009 18:07:07 
To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; 
cing...@uwhealth.org
Subject: RE: [Histonet] Start Up Lab


Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..

 Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 
(lol some labs have a bench area as well as a desk area where food is 
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
cing...@uwhealth.org wrote:

 In the lab?!? For shame. :)

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy
DuBois
 Sent: Wed 7/22/2009 10:29 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Start Up Lab



 And a coffee pot.

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet



 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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Histonet mailing list
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