Re: [Histonet] Suggestions please...
Komal - I once found a lymph node in a roast from the market. A quick "home version" fixative with vinegar and isopropyl kept it good until I could get it into formalin the next day, it cut and demonstrated great. If by chance there is a university with an agriculture program, that could be a source of tissue also, and autolysis could be minimized. Rick T. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Suggestions please...
You could buy some beef liver from the grocery store. Works great. > From: lpw...@sbcglobal.net > To: kjg...@gmail.com; histonet@lists.utsouthwestern.edu > Date: Sun, 23 Oct 2011 08:18:12 -0400 > Subject: Re: [Histonet] Suggestions please... > CC: > > Is there a slaughter house nearby? Call them, and have some documentation > that you are from a university - such as a memo on a letterhead. > > Is there animal research at your university? Can they spare a rat? > > Try to do this right before class, so there is less autolysis. Put tissue in > a plastic bag, and store in refrig for a few hours, until ready. > > Peggy Wenk, HTL(ASCP)SLS > > -Original Message- > From: Komal Gada > Sent: Saturday, October 22, 2011 9:46 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Suggestions please... > > Dear Histonetters, > > I'm currently teaching Histology at a University, and I was hoping for some > suggestions on how to teach students to use a cryostat. I have several > questions: > > 1) Since we do not have access to actual specimens, what would any of you > recommend could be used as a viable option? > > So far, I'm thinking either hot dogs or chicken breasts, but please feel > free to suggest what you think and why so that I can understand the logic. > > 2) Which post-fixative should I use and how long? > > 3) Are there any suggestions for the H&E staining procedure? > > Thanks! > Komal > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Suggestions please...
Is there a slaughter house nearby? Call them, and have some documentation that you are from a university - such as a memo on a letterhead. Is there animal research at your university? Can they spare a rat? Try to do this right before class, so there is less autolysis. Put tissue in a plastic bag, and store in refrig for a few hours, until ready. Peggy Wenk, HTL(ASCP)SLS -Original Message- From: Komal Gada Sent: Saturday, October 22, 2011 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Suggestions please... Dear Histonetters, I'm currently teaching Histology at a University, and I was hoping for some suggestions on how to teach students to use a cryostat. I have several questions: 1) Since we do not have access to actual specimens, what would any of you recommend could be used as a viable option? So far, I'm thinking either hot dogs or chicken breasts, but please feel free to suggest what you think and why so that I can understand the logic. 2) Which post-fixative should I use and how long? 3) Are there any suggestions for the H&E staining procedure? Thanks! Komal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Suggestions please...
I suggest fresh pig-kidney, if you can get it at the butcher. Kidney is relatively easy to cut and you can prepare small quaders of 2-3 mm thickness, that are frozen fast enough directly in the cryocut. After cutting you can wrap it in foil and store it in the fridge (-20°C) for the next students. For teaching I think air drying is good enough before HE-staining. Alternatively you can take 37-40% formaldehyde for a few seconds and rinsing in tapwater afterwards. Then 1 min Harris hematoxylin Rinse in acidified water (dest. water + 1 drop acetic acid) Rinse in tapwater until blue enough Rinse in 96% ethanol 15-30 seconds in 2% eosin in 96% ethanol Rinse in 96% ethanol 2 x rinse in absolute ethanol 1 x rinse in xylol or substitute Coverslipping with resin-media Hope that helps Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Komal Gada Gesendet: Samstag, 22. Oktober 2011 15:46 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Suggestions please... Dear Histonetters, I'm currently teaching Histology at a University, and I was hoping for some suggestions on how to teach students to use a cryostat. I have several questions: 1) Since we do not have access to actual specimens, what would any of you recommend could be used as a viable option? So far, I'm thinking either hot dogs or chicken breasts, but please feel free to suggest what you think and why so that I can understand the logic. 2) Which post-fixative should I use and how long? 3) Are there any suggestions for the H&E staining procedure? Thanks! Komal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Suggestions please...
Dear Histonetters, I'm currently teaching Histology at a University, and I was hoping for some suggestions on how to teach students to use a cryostat. I have several questions: 1) Since we do not have access to actual specimens, what would any of you recommend could be used as a viable option? So far, I'm thinking either hot dogs or chicken breasts, but please feel free to suggest what you think and why so that I can understand the logic. 2) Which post-fixative should I use and how long? 3) Are there any suggestions for the H&E staining procedure? Thanks! Komal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
