To my knowledge it is near impossible to obtain an adequate HE stain
on thick resin sections. If this is not true, I would be interested in
the same information.
Jack
On Apr 3, 2009, at 6:12 PM, Garcia, Lori, Sr. Scientist lori.gar...@medtronic.com
wrote:
This question is specifically for Linda Jenkins or anyone who has
worked
with Technovit 7200,
I am trying to do an HE stain on ~70 um thick ground sections on
plastic slides. I have followed the Donath method all the way through
processing, but the sections do not pick up stains the way the booklet
says they should. I am even having trouble following GMA staining
procedures that I have found, in fact am afraid to try too many
because
the acrylic forms tiny cracks that interfere with microscopy. I have
researched the histonet archives, but haven't found anything other
than
a couple of similar inquiries.
I would very much appreciate any advice from people who have worked
with
this specific formulation.
Happy Friday!
Lori
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