RE: [Histonet] Tissue Shriveling in Paraffin
You could have got the tissue wet or to hydrated. Sometimes after you face the block if you leave it on the ice to long and let moisture get into the tissue then cut the slide, it looks ok at the time, but once the moisture gets back out of the tissue it will start to look concave. If you are going to be leaving your blocks on the ice for a long time just put a 4X4 or paper towel under the block, this helps. Good luck =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle Aono Sent: Friday, June 24, 2011 8:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Shriveling in Paraffin I was cutting some bone/joint tissue and noticed that the cartilaginous portion was concave/indented, instead of flush with the rest of the block surface. Even as I continued to cut that portion always seemed a little sunken into the block face and all the sections crumbled. I didn't seal the block after I was done and when I came back the next day the entire tissue sample was shriveled and pulling away from the paraffin. I'm new, but in the few bone sections I've done I've never had this happen! Any ideas? ~Shelly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Shriveling in Paraffin
I was cutting some bone/joint tissue and noticed that the cartilaginous portion was concave/indented, instead of flush with the rest of the block surface. Even as I continued to cut that portion always seemed a little sunken into the block face and all the sections crumbled. I didn't seal the block after I was done and when I came back the next day the entire tissue sample was shriveled and pulling away from the paraffin. I'm new, but in the few bone sections I've done I've never had this happen! Any ideas? ~Shelly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue Shriveling in Paraffin
Why not try resin embedding techniques? Less shrinkage than paraffin, more specimen/block stability in cutting, no damaging effects of decalcification and a many times you have a better and more clear morphological representation. I could help you to achieve this if interested, so feel free to contact me as needed (31-281-1975). Jack Jack Ratliff Senior Histologist, Biomimetic Therapeutics, Inc. Chair, Hard Tissue Committee - National Society for Histotechnology On Jun 24, 2011, at 8:27 AM, Michelle Aono aono...@auburn.edu wrote: I was cutting some bone/joint tissue and noticed that the cartilaginous portion was concave/indented, instead of flush with the rest of the block surface. Even as I continued to cut that portion always seemed a little sunken into the block face and all the sections crumbled. I didn't seal the block after I was done and when I came back the next day the entire tissue sample was shriveled and pulling away from the paraffin. I'm new, but in the few bone sections I've done I've never had this happen! Any ideas? ~Shelly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Tissue Shriveling in Paraffin
That sounds like insufficient infiltration with paraffin. Melt your block again and let it sit in paraffin for a few hours or even over night. Most time this helps to get better sections. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Michelle Aono Gesendet: Freitag, 24. Juni 2011 15:28 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Tissue Shriveling in Paraffin I was cutting some bone/joint tissue and noticed that the cartilaginous portion was concave/indented, instead of flush with the rest of the block surface. Even as I continued to cut that portion always seemed a little sunken into the block face and all the sections crumbled. I didn't seal the block after I was done and when I came back the next day the entire tissue sample was shriveled and pulling away from the paraffin. I'm new, but in the few bone sections I've done I've never had this happen! Any ideas? ~Shelly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet