[Histonet] Undecalcified bone IHC
Does anyone have a protocol for Undecalcified bone for IHC? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Undecalcified bone IHC
Undecalcified? How are you going to section it? If you can section it, just use any IHC protocol for regular sections. Good luck! René J. --- On Mon, 3/12/12, Jeffery Howery jeffery.how...@jcl.com wrote: From: Jeffery Howery jeffery.how...@jcl.com Subject: [Histonet] Undecalcified bone IHC To: histonet@lists.utsouthwestern.edu Date: Monday, March 12, 2012, 10:59 AM Does anyone have a protocol for Undecalcified bone for IHC? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Undecalcified bone IHC
Hi Jeff, If is it possible a few more specifics of how the tissue has been received, processed and evaluated would help. Undecalcified bone sectioning procedures vary and also what specific markers are you looking to do is important. Vikki On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa rjbu...@yahoo.com wrote: Undecalcified? How are you going to section it? If you can section it, just use any IHC protocol for regular sections. Good luck! René J. --- On Mon, 3/12/12, Jeffery Howery jeffery.how...@jcl.com wrote: From: Jeffery Howery jeffery.how...@jcl.com Subject: [Histonet] Undecalcified bone IHC To: histonet@lists.utsouthwestern.edu Date: Monday, March 12, 2012, 10:59 AM Does anyone have a protocol for Undecalcified bone for IHC? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Undecalcified bone IHC
René Jeffery, I don't see sectioning as that big of a challenge. I often thin section mineralized bone sections ~7 microns with my Leica microtome. Although I have a great interest in developing an IHC technique for mineralized bone, I have never had a spare moment to carry out any developmental work. I am concerned more about the effects of the solvents used in hard tissue histology on the antigens/epitopes of interest. Does anyone use IHC staining on PMMA mineralized bone specimens on a regular basis? Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbr...@andrew.cmu.edu On Mar 12, 2012, at 11:06 AM, Rene J Buesa wrote: Undecalcified? How are you going to section it? If you can section it, just use any IHC protocol for regular sections. Good luck! René J. --- On Mon, 3/12/12, Jeffery Howery jeffery.how...@jcl.com wrote: From: Jeffery Howery jeffery.how...@jcl.com Subject: [Histonet] Undecalcified bone IHC To: histonet@lists.utsouthwestern.edu Date: Monday, March 12, 2012, 10:59 AM Does anyone have a protocol for Undecalcified bone for IHC? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
