Re: [Histonet] Vectastain elite ABC kit- multiple dips ok?

2010-09-29 Thread Geoff McAuliffe 

Teresa Iglesias wrote:

Hi all,
I have three 24-well plates with three brain sections in each well. For IHC,
do I have to prepare three different/fresh ABC dips for each 24-well screen
plate or can I incubate (30min) each successive screen plate into the
already used dip of ABC? 

I would not.

Do I have to leave it in longer to allow adequate
binding?
  
I may be that the ABC has been consumed by the first one or two 24 well 
plates?

I know you can do this with DAB but you have a visual indicator that you've
left it in long enough in that case.
Any experience with this?
  


Why not ask Vector? After all, they made the stuff.

Geoff

Thanks,
-Teresa

  



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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcaul...@umdnj.edu

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[Histonet] Vectastain elite ABC kit- multiple dips ok?

2010-09-28 Thread Teresa Iglesias 
Hi all,
I have three 24-well plates with three brain sections in each well. For IHC,
do I have to prepare three different/fresh ABC dips for each 24-well screen
plate or can I incubate (30min) each successive screen plate into the
already used dip of ABC? Do I have to leave it in longer to allow adequate
binding?
I know you can do this with DAB but you have a visual indicator that you've
left it in long enough in that case.
Any experience with this?
Thanks,
-Teresa

-- 
__
Teresa Iglesias
Graduate Group in Animal Behavior
Department of Evolution and Ecology
University of California-Davis
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